²é¿´: 4399  |  »Ø¸´: 3

252209196

гæ (³õÈëÎÄ̳)

[ÇóÖú] ÖÊÆ×µÄbottom upºÍtop down ÓÐÊ²Ã´Çø±ð°¡

ÔÚ×ö±¨¸æÊ±·¢ÏÖÁËÕâ¸öÎÊÌ⣬¿ÉÒÔ°ïæ½âÊÍÏÂÂð£¿
»Ø¸´´ËÂ¥

» ÊÕ¼±¾ÌûµÄÌÔÌûר¼­ÍƼö

¾«Æ··­Ò뼯½õ

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

lzjessie_wlf

½ð³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
252209196: ½ð±Ò+3, ¡ï¡ï¡ïºÜÓаïÖú, ¿É·ñÔÙ°ïæ˵ÏÂÁ½ÖÖ·½·¨µÄÓÅȱµã£¿ 2012-08-14 18:30:10
Сµ¤Ä¾Ä¾: ½ð±Ò+3, ¹ÄÀø»ý¼«Ó¦Öú£¬½â´ðÎÊÌâ 2012-08-15 15:26:55
˵µÄÊÇproteomicµÄbottom up ô£¿
  bottom upÖ¸µÄÊÇproteins Ïȱ»digestion, È»ºó£¬¶ÔdigestionºóµÄpeptides ·ÖÀë´¿»¯ºó£¬×÷MSMS.  È»ºó£¬ ÓÉMSMSµÄƬ¶ÏÐÅÏ¢´Óµ×¶ËÍÆµ¼³öpepetideµÄsequence£¬ÔÙ½«ÕâЩpeptide°´ÕÕdigestionµÄ¹æÂÉ£¬ÍƶϳöproteinµÄsequence.
  Õâ¸öÒ»°ãµÄMALDI¶¼ÒÑ¿ÉÒÔ±íÕ÷¡£

top down ÔòÊÇÏȶÔproteins ·ÖÀë´¿»¯£¬ ʹÓÃMSÑ¡ÔñÌØ¶¨µÄm/z µÄµ°°×£¬Ö±½Ó¶Ôµ°°××÷MS2, MS3, ¡£¡£¡£¡££¬È»ºóÓÉÕâЩËéÆ¬ÍÆ¶Ïµ°°×µÄ×é³É¡£ÕâÐèÒªÌØÊâµÄÖÊÆ×ÒÇ£¬ESI-FTICR.


http://en.wikipedia.org/wiki/File:Bottom-up_vs_top_down.svg
2Â¥2012-08-14 14:54:24
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

lzjessie_wlf

½ð³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

ÒýÓûØÌû:
2Â¥: Originally posted by lzjessie_wlf at 2012-08-14 14:54:24
˵µÄÊÇproteomicµÄbottom up ô£¿
  bottom upÖ¸µÄÊÇproteins Ïȱ»digestion, È»ºó£¬¶ÔdigestionºóµÄpeptides ·ÖÀë´¿»¯ºó£¬×÷MSMS.  È»ºó£¬ ÓÉMSMSµÄƬ¶ÏÐÅÏ¢´Óµ×¶ËÍÆµ¼³öpepetideµÄsequence£¬ÔÙ½«ÕâЩpeptide°´ÕÕ ...

Á½ÖÖ·½·¨µÄÓŵãÔÚÓÚ¸÷×Ô·ÖÎö»òÕß±íÕ÷µÄÖØµã²»Ò»Ñù°É¡£
"bottom up" ÊÇÔÚpeptide level£¬ "top down" ÊÇintact protein level¡£
"top down" ¿ÉÓÃÀ´Ñо¿ protein conformations, ÉõÖÁ protein complexes£¬ÕâЩ¶¼ÓÐÀûÓÚÑо¿»ò·¢ÏÖ¼²²¡µÄmechanism£¬Ì½Ë÷ijЩ¼²²¡µÄbiomarker.

¡°top down" ÓŵãÊǸßͨÁ¿É¸Ñ¡£¬¼ò»¯ÁËÑùÆ·µÄÖÆ±¸¹ý³ÌºÍÊ¡ÂÔdigestion¹ý³Ì¡£
¡±bottom up" ¾ÍÊÇ·Ö±æÐԸߣ¬¿ÉÒÔͬʱ·ÖÎö¶à¸öµ°°×¡£

¡±bottom up"µÄȱµãÊÇʵÑé²½Öè·±Ëö£¬Êý¾Ý·ÖÎöÁ¿´ó£¬¶ÔÓÚÖ±½ÓÑо¿clinic sample ²»Èçtop down.
¡°top down"¶ÔMSÒÇÆ÷µÄ·Ö±æÂÊÒªÇó¸ß¡£Ò»°ãMALDIµÄ·Ö±æÂÊ´ó¸ÅÊÇ10e4-1e5 (20000-50000), ESI ÊÇ10e3-10e4 (5000), FTICR £¨>500000).

¾ßÌåµÄÓÅȱµã²Î¿¼ÒÔÏÂÎÄÏס£

http://www.bio-rad.com/webroot/w ... /Bulletin_5908A.pdf
http://www.advion.com/events/documents/Advion_webinar_031311.pdf
http://onlinelibrary.wiley.com/doi/10.1002/mas.20015/pdf
3Â¥2012-08-14 22:42:04
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

lzjessie_wlf

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
3Â¥: Originally posted by lzjessie_wlf at 2012-08-14 22:42:04
Á½ÖÖ·½·¨µÄÓŵãÔÚÓÚ¸÷×Ô·ÖÎö»òÕß±íÕ÷µÄÖØµã²»Ò»Ñù°É¡£
"bottom up" ÊÇÔÚpeptide level£¬ "top down" ÊÇintact protein level¡£
"top down" ¿ÉÓÃÀ´Ñо¿ protein conformations,  ...

¸üÕý£º
FTICR £¨>50000)
4Â¥2012-08-15 20:53:07
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ 252209196 µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 0703 ÎïÀí»¯Ñ§µ÷¼Á +3 ÎÒ¿ÉÒÔÉϰ¶µÄ¶Ô 2026-03-13 5/250 2026-03-16 10:50 by ÎÒ¿ÉÒÔÉϰ¶µÄ¶ÔÂ
[¿¼ÑÐ] 308Çóµ÷¼Á +3 ÊÇLupa°¡ 2026-03-16 3/150 2026-03-16 10:07 by Çóµ÷¼Ázz
[¿¼ÑÐ] Áº³ÉΰÀÏʦ¿ÎÌâ×é»¶Ó­ÄãµÄ¼ÓÈë +6 һѼѼӴ 2026-03-14 7/350 2026-03-15 22:12 by Winj1e
[¿¼ÑÐ] 274Çóµ÷¼Á +4 ʱ¼äµã 2026-03-13 4/200 2026-03-15 15:29 by Rambo13
[¿¼ÑÐ] 328Çóµ÷¼Á +3 5201314Lsy£¡ 2026-03-13 6/300 2026-03-14 15:31 by hyswxzs
[¿¼ÑÐ] 306Çóµ÷¼Á +4 ÌÆÞ²Þ² 2026-03-09 4/200 2026-03-14 01:19 by JourneyLucky
[¿¼ÑÐ] 265Çóµ÷¼Á +9 Сľ³æ085600 2026-03-09 12/600 2026-03-14 01:11 by JourneyLucky
[¿¼ÑÐ] Ò»Ö¾Ô¸°²»Õ´óѧ²ÄÁϹ¤³Ìר˶313·Ö£¬Çóµ÷¼ÁµÄѧУ +8 YuÏÈÉú 2026-03-10 10/500 2026-03-14 01:04 by JourneyLucky
[¿¼ÑÐ] 308Çóµ÷¼Á +5 ÊÇLupa°¡ 2026-03-11 5/250 2026-03-13 22:13 by JourneyLucky
[¿¼ÑÐ] Çó²ÄÁϵ÷¼Á +5 ¸ô±Ú³ÂÏÈÉú 2026-03-12 5/250 2026-03-13 22:03 by ÐÇ¿ÕÐÇÔÂ
[˶²©¼ÒÔ°] 085600 260·ÖÇóµ÷¼Á +3 Ìì¿Õ»¹ÏÂÓêô 2026-03-13 5/250 2026-03-13 18:46 by Ìì¿Õ»¹ÏÂÓêô
[¿¼ÑÐ] Çóµ÷¼Á +5 Ò»¶¨ÓÐѧÉÏ- 2026-03-12 5/250 2026-03-13 18:31 by ms629
[¿¼ÑÐ] Çóµ÷¼Á +7 18880831720 2026-03-11 7/350 2026-03-13 16:10 by JourneyLucky
[¿¼ÑÐ] 295Çóµ÷¼Á +3 Сذ×ÐÖ­ 2026-03-12 3/150 2026-03-13 15:17 by vgtyfty
[¿¼ÑÐ] 285Çóµ÷¼Á +4 ytter 2026-03-12 4/200 2026-03-13 14:48 by jxchenghu
[¿¼ÑÐ] 085600²ÄÁÏÓ뻯¹¤ 309·ÖÇëÇóµ÷¼Á +7 dtdxzxx 2026-03-12 8/400 2026-03-13 14:43 by jxchenghu
[¿¼ÑÐ] Çóµ÷¼Á ×ÊÔ´Óë»·¾³ 285 +3 δÃû¿¼Éú 2026-03-10 3/150 2026-03-13 10:31 by houyaoxu
[¿¼ÑÐ] Ñо¿ÉúÕÐÉú +3 Ð캣ÌÎ11 2026-03-10 7/350 2026-03-12 14:26 by Ð캣ÌÎ11
[»ù½ðÉêÇë] Ìá½»ºóµÄ»ù½ð±¾×Ó£¬ÒÑÈÃѧУ³·»ØÁË£¬¿É·ñ»»¿Ú×ÓÌá½» +3 dut_pfx 2026-03-10 3/150 2026-03-11 08:38 by kudofaye
[¿¼ÑÐ] 085602»¯¹¤Çóµ÷¼Á +7 ¶­boxing 2026-03-10 7/350 2026-03-10 17:07 by BruceLiu320
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û