在一篇文章里看到这么一段描述(这段话背景是测药品中组分,内标物internal standard用的是药品的同位素内标)
Recoveries of SPE process was determined by spiking samples of synthetic solutions with analytes (50 µg L-1) and internal standards (50 µg L-1). The samples were replicated six times. The absolute recoveries of analytes were determined in comparison with standard solutions; the relative recoveries were determined by correcting the absolute recoveries with the recovery of the internal standards.
请问高人,这个相对回收率和绝对回收率怎么计算?
希望给出计算公式等
因为标准曲线standard curve已经做出来了。标准曲线不经过固相萃取SPE。