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[求助]
16s 序列上传NCBI的问题
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If the sequence was obtained from a cultured isolate, then a strain identifier is required. 此处strain identifier指的是什么？ 谢谢！！ 详情如下： If the sequence was obtained from a cultured isolate, then a strain identifier is required. (A strain identifier is not the same as a species epithet. The proper species name of your bacterial isolate is not required.) Strain identifiers distinguish specific cultures so a historical continuity can be traced from the parent culture to any subsequent subculture(s). This is important when strains differ at an infraspecies level, or, in some cases, when they have been misidentified and are consequently reclassified in another species. The strain designation you provide will serve to distinguish yours from other extant and future isolates that might be obtained elsewhere. Your isolates do not need to be deposited in a culture collection in order to have strain designations. A strain may be designated in any manner: by the name of an individual or locality, or by a string of numbers and/or letters. For example, how do you distinguish this culture in your laboratory notebook? Alternatively, if this sequence was obtained by direct PCR-amplification and cloning of an environmental bulk DNA sample, then please provide the name of the clone or laboratory identifier. |
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2楼2011-12-10 07:35:17
