| ²é¿´: 519 | »Ø¸´: 2 | |||
| ±¾Ìû²úÉú 1 ¸ö BioEPI £¬µã»÷ÕâÀï½øÐв鿴 | |||
[½»Á÷]
һС¶ÎÎÄÏ×Öз½·¨µÄÎÊÌ⣿£¿£¿Óеãû¿´Ã÷°×
|
|||
|
±¾ÈËÐÂÊÖ£¬ÔÚһƪÎÄÏ×ÉÏѧϰһ¸öµ°°×µÄ´¿»¯²½Ö裬Õâ¸öµ°°×ÓõÄÊÇTEV-HIS TAG , ¶øÇÒ»¹ÓÐGST±êÇ©£¬ÎÒ²»´óÃ÷°×£¬ÎªÊ²Ã´Õâô·±Ëö£¬Ö»ÓÃHIS»òÕßGST²»ÐÐô£¿Ò»ÏÂÊÇÕâ¶ÎµÄÔÎÄ The supernatant was loaded onto a glutathione sepharose column (GE Healthcare). After extensive washing with 20mM Tris buffer pH 8.0, 500mM NaCl, 1mM DTT, the GSTmoiety was cleaved by His-tagged TEV protease in the same wash buffer at 4¡ãC overnight. The protein was passed through a Ni2+- NTA agarose column (Qiagen) to eliminate the His-tagged TEV protease, |
» ²ÂÄãϲ»¶
291Çóµ÷¼Á
ÒѾÓÐ12È˻ظ´
317Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
0703»¯Ñ§Çóµ÷¼Á£¬¸÷λÀÏʦ¿´¿´ÎÒ£¡£¡£¡
ÒѾÓÐ3È˻ظ´
339Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
287Çóµ÷¼Á
ÒѾÓÐ8È˻ظ´
Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ 070300 ѧ˶ 336·Ö Çóµ÷¼Á
ÒѾÓÐ8È˻ظ´
085602 »¯¹¤×¨Ë¶ 338·Ö Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
0856£¬²ÄÁÏÓ뻯¹¤321·ÖÇóµ÷¼Á
ÒѾÓÐ4È˻ظ´
Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ²ÄÁÏÓ뻯¹¤ 264·Ö¸÷¿Æ¹ýAÇø¹ú¼ÒÏß
ÒѾÓÐ10È˻ظ´
266Çóµ÷¼Á
ÒѾÓÐ8È˻ظ´
» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:
¹ØÓڲο¼ÎÄÏ×ÖеÄÒ»µãÎÊÌâ
ÒѾÓÐ1È˻ظ´
¹ØÓÚÓ¢ÎÄÎÄÏ×ÖаÍÎ÷×÷ÕßÃû×ÖµÄÐÕºÍÃûµÄÎÊÌâ
ÒѾÓÐ5È˻ظ´
ÖØ½ðÐüÉÍ£ºÎÄÏ×Öеļ¸¶Î»°·Òëһϣ¨ÒëÒ»¶Î¸øÒ»·Ý£©
ÒѾÓÐ8È˻ظ´
ÎÄÏ×ÖÐÓо仰²»Ì«Ã÷°×£¡£¡Íû´ó¼ÒÖ¸µã£¡~~¶àл¶àлÀ²
ÒѾÓÐ3È˻ظ´
ÇóÖúÒ»ÎÄÏ×Öо²µçÄܵļÆËãµÄÎÊÌâ
ÒѾÓÐ4È˻ظ´
ÔĶÁÓ¢ÎÄÎÄÏ×ÖеÄÎÊÌâ
ÒѾÓÐ5È˻ظ´
ÓйØÍ¿²ãÎÄÏ×ÖС°etching¡± Ò»´ÊµÄ·ÒëÎÊÌâ¡£¡£¡£¼±£¡£¡
ÒѾÓÐ15È˻ظ´
ÎÄÏ×ÖÐһЩÎÊÌâ²»Ã÷°×¸øÍ¨ÐÅ×÷ÕßдÐŲ»»Ø
ÒѾÓÐ6È˻ظ´
¡¾ÇóÖú¡¿¿´ÎÄÏ×ÖÐÓöµ½Ò»¸öÎÊÌ⣬Çë½Ì¸÷λ´óϺ!
ÒѾÓÐ10È˻ظ´
¡¾ÇóÖú¡¿¹ØÓÚÕâÆªÎÄÏ×ÖÐSOC-OCVÇúÏßµÄÎÊÌâ
ÒѾÓÐ8È˻ظ´
» ÇÀ½ð±ÒÀ²£¡»ØÌû¾Í¿ÉÒԵõ½:
ÄÍÈÈÑõ»¯ÄÍÈȻƱäЧ¹ûºÃµÄ¾ÛÃѶàÔª´¼ÖƱ¸µÄ¾Û°±õ¥¶¼ÓÐÄÄЩDZÔÚÓ¦ÓÃÁìÓò£¿
+2/180
µçÁ¦È«¹úÖØµãʵÑéÊÒ˫һÁ÷AÀ೤½Ñ§ÕßÍŶÓÕÐ2026ÄêÈ«ÈÕÖÆ²©Ê¿1Ãû£¨4ÔÂ3ÈÕ½ØÖ¹£©
+2/76
Áôѧ--²©Ê¿ÕÐÉú
+1/51
¹ú¼Ò¹¤³ÌÎïÀí½»²æ¿ÆÑ§Ñо¿ÖÐÐÄÕÐÊÕÄý¾Û̬ÎïÀí¼°Ïà¹Ø×¨ÒµË¶Ê¿Ñо¿ÉúÒ»Ãû
+1/46
»¶Ó»¯Ñ§¡¢ÉúÎïÓëÒ½Ò©¡¢Ò©Ñ§£¬ÉúÎʳƷµÈÏà¹Ø×¨ÒµµÄͬѧ
+1/39
ÖÐÔʳƷʵÑéÊÒ & Ö£ÖÝÇṤҵ´óѧ2026Äê˶ʿÑо¿ÉúÁªºÏÅàÑøµ÷¼Á¹«¸æ
+1/39
´ºÃß²»¾õÏþ
+1/19
Î人¹¤³Ì´óѧ×ÊÔ´ÉúÎï¼¼Êõ¿ÎÌâ×éÉúÎï/»·¾³/»¯¹¤/¿óÎïרҵ˶ʿ¡¢²©Ê¿Ñо¿ÉúÕÐÉú¼°µ÷¼Á
+1/17
±±¾©Ê¯ÓÍ»¯¹¤Ñ§ÔºÖÇÄÜ·À¸¯Í¿²ã¿ÎÌâ×éÕÐÊÕ2026¼¶µ÷¼ÁÑо¿Éú
+1/16
˫һÁ÷´óÑ§ÏæÌ¶´óѧ¡°»¯¹¤¹ý³ÌÄ£ÄâÓëÇ¿»¯¡±¹ú¼ÒµØ·½ÁªºÏ¹¤³ÌÑо¿ÖÐÐÄÕÐÊÕ¸÷À಩ʿÉú
+1/16
»ªÇÈ´óѧ·ÂÉú¶à³ß¶È¿×µÀ¿ÎÌâ×é·Ö²¿ÁªºÏ985ÍŶÓÕвÄÁÏ¡¢»¯Ñ§½Ìʦ¡¢²©Ê¿ºó¡¢Ë¶²©Ñо¿Éú
+2/16
26ÄêÉ격×Ô¼ö-¼ÆËã»úÊÓ¾õ
+1/11
ÖйúµØÖÊ´óѧ£¨±±¾©)¹¤³Ì¼¼ÊõѧԺ°²ÀÏʦ×éÕÐÊÕ2026µ÷¼ÁÉú
+1/11
²©Ê¿ÕÐÉú | Çൺ¿Æ¼¼´óѧ£¨¸ß·Ö×Ó¡¢»¯Ñ§¡¢²ÄÁÏ¡¢Á¦Ñ§¡¢»úµç¹¤³Ì¡¢¼ÆËã»ú·ÂÕæ£©
+1/10
³ÏÖ¿ÕÐÉú£¨Ë¶Ê¿+²©Ê¿£©
+1/9
Öйú¿ÆÑ§ÔºÉϺ£¹èËáÑÎÑо¿ËùÕÐÆ¸¿ÆÑÐÖúÀí£¨ÄÉÃײÄÁÏÓëÉúÎïÓ¦Ó÷½Ïò£©
+1/8
Ò»Ö¾Ô¸Öйúº£Ñó´óѧ095137£¬³õÊԳɼ¨312£¬²»ÌôѧУºÍµØÇø£¬ÓÐѧÉϾÍÐУ¡
+1/6
»¶Ó¸÷λͬѧÉêÇë²Î¼Ó°ÄÃÅ´óѧ½¡¿µ¿ÆÑ§Ñ§ÔºµÄ2026ÄêÏÄÁîÓª£¬±¨Ãû½ØÖ¹4ÔÂ12ºÅ
+1/6
»ùÓÚ»úÆ÷ѧϰºÍÈ˹¤ÖÇÄܵĽṹ/ºÏ½ðÉè¼Æ-Î÷°²½»´ó
+1/5
Çóµ÷¼Á
+1/2
cloverplm
½ð³æ (СÓÐÃûÆø)
- BioEPI: 1
- Ó¦Öú: 7 (Ó×¶ùÔ°)
- ½ð±Ò: 699.7
- Ìû×Ó: 199
- ÔÚÏß: 52.9Сʱ
- ³æºÅ: 1343320
¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
silicare(½ð±Ò+5, BioEPI+1): 2011-08-10 12:05:50
loveskyzhou(½ð±Ò+2): лл 2011-08-10 12:55:27
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
silicare(½ð±Ò+5, BioEPI+1): 2011-08-10 12:05:50
loveskyzhou(½ð±Ò+2): лл 2011-08-10 12:55:27
|
Ö±Òëһϣº ·Ö×°ÉÏÇåÖÁ¹Èë׸ÊëÄÊÕ¼¯Öù(GE Healthcare)£¬ÓÃ20mM Tris buffer pH 8.0, 500mM NaCl, 1mM DTT³ä·ÖÏ´µÓºó£¬ÔÙÓÃÏàͬµÄbuffer4¡æ½þÅݹýÒ¹£¬²¿·ÖGST±»His-tagged TEV µ°°×ø¼ôÇС£Ëæºó¸Ãµ°°×ͨ¹ýNi2+- NTA ÇíÖ¬ÌÇ·ÖÀëÖù£¨Qiagen£©È¥³ýHis-tagged TEV µ°°×ø¡£ |
2Â¥2011-08-09 20:58:15
imfly77
ľ³æ (СÓÐÃûÆø)
- BioEPI: 2
- Ó¦Öú: 4 (Ó×¶ùÔ°)
- ½ð±Ò: 3065.8
- Ìû×Ó: 287
- ÔÚÏß: 151.3Сʱ
- ³æºÅ: 1324638
¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
adslye(½ð±Ò+5): ½âÊ͵غÜÇå³þÁË£¬¸Ðл½»Á÷£¡ 2011-08-29 09:53:30
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
adslye(½ð±Ò+5): ½âÊ͵غÜÇå³þÁË£¬¸Ðл½»Á÷£¡ 2011-08-29 09:53:30
|
¼òµ¥µÄ˵£¬£¨Ä¿µÄµ°°×-GST£©Ê×ÏȽáºÏÓÚglutathione sepharose column£¬È»ºóͨ¹ý¼ÓÈëHis-tagged TEV protease £¨µ°°×ø£©ÇжÏÄ¿µÄµ°°×ºÍGSTÖ®¼äµÄÁ¬½ÓëÄ£¬ÔÙ¼ÓÈëbuffer¾Í¿ÉÒÔ°ÑÄ¿µÄµ°°×Ï´ÍÑÏÂÀ´£¬Öù×ÓÉÏÔòÊ£Óà½áºÏµÄGST¡£ Ï´ÍѳöµÄÄ¿µÄµ°°×ÀïÃæ»¹ÓÐHis-tagged TEV proteaseÔÓÖÊ£¬Õⲿ·Öͨ¹ýNiÖùºÍHis-tagged TEV proteaseÌØÒìÐÔ½áºÏ¶øÈ¥µô£¬×îºóÈÜÒºÖоÍÖ»ÓÐÄ¿µÄµ°°×ÁË¡£ Õâ¸ö·½·¨ÊÇĿǰÓõıȽ϶àµÄÒ»ÖÖ´¿»¯·½·¨£¬µ«Í¨¹ýÕâЩÇ׺ÍÎü¸½Ö®ÀàµÄ°ì·¨´¿»¯µ°°×ÖÊ£¬×îºó¶¼±ØÐ뽫ÖîÈçHistag¡¢GSTºÍCBDÖ®ÀàµÄ±ê¼ÇtagÈ¥µô¡£µ°°×ø·½·¨ÊÇʵÑéÊÒÀï³£ÓõÄÒ»ÖÖ¡£ÁíÍâÒ²ÓаüÀ¨»¯Ñ§·¨£¨ÀýÈçôǰ·ºÍä廯Ç⣩¡¢ÄÚº¬ëÄ£¨intein£©Ö®ÀàµÄµÈµÈÆäËûÊֶΡ£ |
3Â¥2011-08-10 15:27:51














»Ø¸´´ËÂ¥