24小时热门版块排行榜

返回列表

当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖

huofuman

木虫 (小有名气)

应助: 1 (幼儿园)
金币: 1456.2
帖子: 116
在线: 99.6小时
虫号: 268046

[交流] 【求助/交流】5‘UTR的问题

求教于高手
我刚做了个5’RACE，将得到的5‘UTR序列与之前通过TAIL-PCR得到了启动子上游序列进行比对，却发现5‘UTR序列和启动子序列毫无同源性。
难道mRNA的加工导致5‘UTR与其在基因组的序列完全不同了吗？

回复此楼

» 猜你喜欢

» 本主题相关价值贴推荐，对您同样有帮助:

关于雅思5.5的问题。已经有22人回复
幼稚问题一个，在Genbank上查某基因，为何会出现两个mRNA，两个CDS区？已经有12人回复
MS 5.0 安装问题已经有3人回复
计划书：购置与试制仪器设备在 5 万元以上问题请教已经有27人回复
【求助完结】Linux系统下MS5.5的并行计算调度问题已经有12人回复
【求助】关于ms5.5的问题已经有16人回复
【求助/交流】请问高手，怎么确定一个基因的5‘UTR和3'UTR，感谢啦！已经有5人回复
【求助】嘧啶环5位氟化的问题已经有8人回复
【求助/交流】仍然是关于引物的一个问题！！！已经有4人回复
【求助/交流】从RNA到cDNA合成再到克隆PCR的实验技巧和操作细节问题若干，寻求解答已经有12人回复
【求助】关于HZSM-5分子筛的问题已经有13人回复

» 抢金币啦！回帖就可以得到:

查看全部散金贴

1楼 2011-04-02 14:57:35

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

5‘UTR是5’非翻译区域的意思。
下面是从维基百科粘过来的。
Five prime untranslated region
From Wikipedia, the free encyclopediaJump to: navigation, search
A messenger ribonucleic acid (mRNA) molecule codes for a protein through translation. The mRNA also contains regions that are not translated: in eukaryotes these include the 5' untranslated region, 3' untranslated region, 5' cap and poly-A tail.

The five prime untranslated region (5' UTR), can contain elements for controlling gene expression by way of regulatory elements. It begins at the transcription start site and ends one nucleotide (nt) before the start codon (usually AUG) of the coding region. In prokaryotes, the 5' UTR usually contains a ribosome binding site (RBS), also known as the Shine Dalgarno sequence (AGGAGGU).

The 5' UTR has a median length of ~150 nt in eukaryotes, but can be as long as several thousand bases. Some viruses and cellular genes have unusually long and structured 5' UTRs which may impact gene expression. On average, 3' UTR tend to be twice as long as the 5' UTR [1]. In prokaryotic mRNAs the 5' UTR is normally shorter.

mRNA structure, approximately to scale for a human mRNA, where the median length of the 5'UTR is 170 nucleotidesSeveral regulatory sequences may be found in the 5' UTR:

Binding sites for proteins, that may affect the mRNA's stability or translation, for example iron responsive elements, that regulate gene expression in response to iron.
Riboswitches.
Sequences that promote or inhibit translation initiation.
Introns within 5' UTRs have been linked to regulation of gene expression and mRNA export