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[交流] 【求助/交流】5‘UTR的问题

求教于高手
我刚做了个5’RACE，将得到的5‘UTR序列与之前通过TAIL-PCR得到了启动子上游序列进行比对，却发现5‘UTR序列和启动子序列毫无同源性。
难道mRNA的加工导致5‘UTR与其在基因组的序列完全不同了吗？

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1楼2011-04-02 14:57:35

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

5‘UTR是5’非翻译区域的意思。
下面是从维基百科粘过来的。
Five prime untranslated region
From Wikipedia, the free encyclopediaJump to: navigation, search
A messenger ribonucleic acid (mRNA) molecule codes for a protein through translation. The mRNA also contains regions that are not translated: in eukaryotes these include the 5' untranslated region, 3' untranslated region, 5' cap and poly-A tail.

The five prime untranslated region (5' UTR), can contain elements for controlling gene expression by way of regulatory elements. It begins at the transcription start site and ends one nucleotide (nt) before the start codon (usually AUG) of the coding region. In prokaryotes, the 5' UTR usually contains a ribosome binding site (RBS), also known as the Shine Dalgarno sequence (AGGAGGU).

The 5' UTR has a median length of ~150 nt in eukaryotes, but can be as long as several thousand bases. Some viruses and cellular genes have unusually long and structured 5' UTRs which may impact gene expression. On average, 3' UTR tend to be twice as long as the 5' UTR [1]. In prokaryotic mRNAs the 5' UTR is normally shorter.

mRNA structure, approximately to scale for a human mRNA, where the median length of the 5'UTR is 170 nucleotidesSeveral regulatory sequences may be found in the 5' UTR:

Binding sites for proteins, that may affect the mRNA's stability or translation, for example iron responsive elements, that regulate gene expression in response to iron.
Riboswitches.
Sequences that promote or inhibit translation initiation.
Introns within 5' UTRs have been linked to regulation of gene expression and mRNA export

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