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caojun2318

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专业: 细胞生物学研究中的新方法

[交流] 【求助/交流】细胞荧光标记问题求助已有2人参与

我想对不同细胞跨膜途径的蛋白（网格蛋白，陷穴蛋白）进行荧光标记，但是查了很久没有查到有哪种特异的荧光标记物能对这两种蛋白进行标记的，有谁做过，或者知道这方面的工作？选用哪些荧光探针能特异标记这些蛋白？

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1楼 2010-11-26 12:45:57

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小木虫(金币+0.5):给个红包，谢谢回帖交流

上海生工有进口试剂盒供应！

HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine)
蛋白罗丹明标记试剂盒
Description:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.(5/6) TAMRA absorbs green visible light at 546nm and emits an orange-red visible light at a maximum emission of 575nm.The NHS ester group provides the simplest and most commonly used group for labeling proteins. The succinimidyl ester group reacts with primary amines in lysine side chains and N-terminal amines forming a stable, covalent amide bond.This kit utilizes Tube-O-REACTOR™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort. The (5/6) TAMRA-SE labeling reagent is also available in our single use OneQuant™ format.
Features:
Dye preweighed and supplied in single use vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time and no protein loss.application for Labeling of proteins, peptides and nucleic acids with a red fluorescent dye.;Suitable for antibody labeling.Store: -15～-20℃
编号包装价格产地
786-058 5 Reactions 2,486 GBios

HOOK™ Dye Labeling Kit (FITC)
蛋白FITC荧光素标记试剂盒
Description:
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 520nm, respectively, therefore emitting green visible light.This kit utilizes Tube-O-Reactor™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort.
Features:
Dye preweighed and supplied in single use vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time.application for Labeling of a green fluorescent dye to proteins and peptides. And Suitable for antibody labeling. Store: -15～-20℃
编号包装价格产地
786-059 5 Reactions 2,486 GBios