| 查看: 365 | 回复: 0 | |||
[交流]
【求助/交流】求助一篇关于反转录转座子复制机制的英文文献
|
|
求助这篇文献，不知道哪里能下载下来，希望哪位高手能帮一下忙，不胜感激！MECHANISMS OF RETROTRANSPOSON REPLICATION Abram Gabriel, Professor Molecular Biology And Biochemrutgers The St Univ Of Nj New Brunswick asb Iii new Brunswick, Nj 08901 Grant 1R01GM060534-01A2 from National Institute Of General Medical Sciences IRG: MBC Abstract: LTR-containing retrotransposons and retroviruses represent two related branches on the phylogenetic tree of reverse transcriptase (RT)-containing mobile genetic elements. Both types of elements use the RNA-dependent DNA polymerase, RT, to replicate their RNA genomes into double strand DNA which is then inserted into a host genome. The movement of retrotransposons within a genome can be harmful, resulting in genomic instability, cancer, sterility and mutations. On the other hand retrotransposons actively contribute to the evolution of their hosts´ genomes. We have a long term interest in the replication mechanisms utilized by retrotransposon Tyl to maintain itself as an endogenous family of mobile elements in its host organism Saccharomyces cerevisiae. Tyl is a particularly useful experimental system to study transposition because it is so easily manipulated, because its natural host is such a genetically tractable organism, and because the steps in its replication are so closely related to the steps involved in retroviral replication. Since DNA polymerases replicate and transmit vital genetic information, the fidelity of their synthesis is a crucial biochemical determinant of their functional capacity. We have found that Tyl RT is an error prone polymerase, that generates errors at specific sites by a novel mechanism. We propose (Aim 1) to explore this new mutagenic mechanism genetically and biochemically, and determine the consequences of this process to Tyl replication fitness. Further, we will identify and characterize additional mutagenic mechanisms employed by Tyl RT during replication. We have created and are characterizing a novel mutation in the active site of Tyl RT which can still synthesize DNA but is blocked for transposition. This defect is suppressible by mutations in both the RNase H and polymerase domains of RT. We plan (Aim 2) to use this novel mutant to examine the distinctions between polymerization and replication, and more broadly, to understand the interactions between protein domains during Tyl replication. Further we will determine whether the properties of this novel mutant are generalizable to the ubiquitous family of retrotransposons whose RTs have not previously been studied Keywords: Retroviridae, host organism interaction, nucleic acid repetitive sequence, transposon /insertion element, virus replication RNA directed DNA polymerase, enzyme activity, gene mutation Saccharomyces cerevisiae, autoradiography, bacteriophage M13, gel filtration chromatography, gene complementation, ion exchange chromatography, polymerase chain reaction, site directed mutagenesis Project start date: 2001-05-01 |
回复此楼» 猜你喜欢
一志愿北交大材料工程总分358求调剂
已经有9人回复
-
286求调剂
已经有11人回复
-
求调剂！生物与医药专硕
已经有6人回复
-
362求调剂一志愿中国石油大学
已经有4人回复
-
085600，321分求调剂
已经有14人回复
-
材料调剂
已经有4人回复
-
一志愿生物与医药，296分，求调剂
已经有11人回复
-
346分的生物与医药08600求调剂
已经有7人回复
-
071000生物学调剂
已经有6人回复
-
一志愿郑州大学材料与化工085600，求调剂
已经有25人回复
找到一些相关的精华帖子，希望有用哦~
急求：矿类的论文发国内或国外哪些英文期刊比较好？
已经有7人回复
- 谋求毕业后回国发展，想投一个SSCI的英文杂志，求推荐！
已经有6人回复
- 求助：中文翻译英文
已经有1人回复
- 请问什么是dislocation ensemble？英文文献老碰到
已经有5人回复
- 求助 英文翻译
已经有2人回复
- 求助翻译成英文 谢谢啦
已经有1人回复
- 【探索生命起源】《深奥的简洁：从混沌，复杂到地球生命的起源》英文文字版[EPUB]
已经有37人回复
- 求助：中文翻译英文，急用，谢谢！！
已经有1人回复
- 求助高手帮忙翻译一小段英文！！！
已经有1人回复
- 求助：英文翻译中文，急用，谢谢
已经有1人回复
- 求翻译部分化学相关的英文文献 能翻译多少是多少
已经有2人回复
- 求氨基酸螯合微量元素方面的英文文献
已经有4人回复
- 求助有关膜片钳的英文翻译
已经有4人回复
- 求助生物化学专业一篇英文文献若干句子的中文翻译
已经有3人回复
- 急！！！！求助一篇中英文对照的文献
已经有3人回复
- 求助英文医学文献一篇
已经有3人回复
- 求助翻译【糖化酶】英文文献一篇
已经有12人回复
点击这里搜索更多相关资源科研从小木虫开始，人人为我，我为人人
10