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For electrophysiological analysis,MCF-7 cells were cultured in 35 mm
petri dishes.Electrophysiological signals were recorded,amplified,
digitized,and analyzed with the use of an Axopatch 200B patch-clamp
(Axon Instruments,Burlingame,CA)in conjunction with Labmaster
hardware(Digidata 1200,Axon Instruments)and Pclamp software(ver
6.03,Axon Instruments).The whole-cell mode of the patch-clamp
technique was used with 4¨C7 MV resistance borosilicate fire-polished
pipettes(Hirschmann
1
,Laborgerate,Eberstadt,Germany).Seal
resistance was typically in the 10¨C20 GV range.After formation of a
seal,the amplifier was switched to voltage-clamp mode and currents
attained stable values within 3¨C5 min.All recordings were made
without leak subtraction.Results were expressed using current-
densities(pA/pF)instead of current amplitude.The cell surface of the
MCF-7 cells was thus estimated by measuring their membrane
capacitance(20.8 4.7 pF,n?63).Whole-cell current was elicited by
voltage ramp or by voltage step from 80 tot80 mV delivered at
30 sec intervals for 250 msec,applied from a holding potential of 40
mV.The cell under investigation was continuously superfused with
control or test solutions.

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whitney1989: ½ð±Ò+12, ·­ÒëEPI+1, ¡ïÓаïÖú 2012-04-23 09:21:53
Mally89: ½ð±Ò+1 2012-05-07 16:56:44
sltmac: ½ð±Ò+4 2012-05-11 08:06:18
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whitney1989

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2Â¥: Originally posted by _¨ŠÈô¡§|ÏàÒÀ at 2012-04-23 00:02:37:
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3Â¥: Originally posted by whitney1989 at 2012-04-23 09:35:48:
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sltmac: ½ð±Ò+4 2012-05-11 08:06:30
80 tot80 mV delivered at
30 sec intervals for 250 msec,
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5Â¥2012-04-23 10:55:37
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