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ansrang

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[交流] 请问这个审稿回复是什么意思？已有14人参与

提了几个问题，最后一个问题是：English language is correct in use and grammar.
这到底是什么意思？是说要我再修改一下语言吗？

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1楼 2010-06-20 02:53:33

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

ansrang

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引用回帖:

Originally posted by ljhcumt at 2010-06-20 07:05:40:
能不能把审稿回复的全文贴一下，这个看不明白

审稿回复如下：
We have received reviewer's reports on your above manuscript. They indicated that it is not acceptable for publication in its present form. However, if you can suitably address the reviewers' comments (included below), I invite you to revise and resubmit your manuscript within 30 days. If you are submitting a revised manuscript

reviewer1#省略。。。
reviewer2#
1) The activities reported in table 1 are by mg. It is not clear if it is mg of CLEA or mg of protease. In the last case, there is no explicit explanation in the experimental part about how the weight of protease in the p-CLEAs or in the CLEas was obtained. No report of procedure to know about remaining protease in the supernatant of the preparation or in the washing steps. The initial amount was 5 mg of papain in 10 mls.
I recommend the authors to be very careful with the methods of detection of enzyme amount as protein quantification. Further material
Bradley J.S.C. Olson and John Markwell
Current Protocols in Protein Science (2007) Supplement 48 ; 3.4.1 to 3.4.29 by John Wiley & Sons, Inc.

2) It is known today that several protein detection methods have several problems in its application to protease/other enzymes concentration-especially if stirring is involved and buffer is present.It is included the parameter Ew as enzyme amount in page 4,but it is not clear how this amount was obtained- if it is protease. IF the reported weight is of CLEA and no mass balance was done to know about how much protease is present in the CLEA, this should be clearly stablished. One way to do it is to measure activity in supernatants and washings,assuming similar activity of the free enzyme and of the remaining not-precipitated and not cross-linked enzyme.

3) Taking into account the above, the authors presented in Table 1 several results associated to their CLEAs. Without the knowledge on the total amount of protease, they do not know per mg of it the actual activity. However, the comparison is pertinent between CLEAs.Without knowledge on the protease amount present in both CLEAs what is not pertinent is the comparison with the free enzyme.

4) With these ideas in mind, how do they know if the lower activity in the case of the CLEA in table 1 is due to the presence of the starch and a "dilution" effect of the starch. Because it is not clear if the activity reports are presented by mg of enzyme or mg of CLEA, this is not known. BTW, if it is by mg of enzyme, how the protease amount in CLEAs was determined?

5) IF the porous structure is different, the authors could try to perform a BET Area determination for the CLEA and the p-CLEA to give further support to their assumptions. They also could perform the complete N2 isotherm to know about the pore distribution.

6)English language is correct in use and grammar.

最后一个问题就是我说的，让人摸不着头脑，请大家指点