| 查看: 1613 | 回复: 14 | |||
muchong5577金虫 (正式写手)
|
[交流]
【求助/交流】脂肪酶的温度已有5人参与
|
| 脂肪酶的最适酶促温度是多少？目前发现的脂肪酶在低温和高温下有活性的极端温度是多少？ |
回复此楼» 猜你喜欢
三无产品还有机会吗
已经有4人回复
-
投稿返修后收到这样的回复，还有希望吗
已经有7人回复
-
压汞仪和BET测气凝胶孔隙率
已经有4人回复
-
博士申请都是内定的吗？
已经有14人回复
-
谈谈两天一夜的“延安行”
已经有13人回复
-
氨基封端PDMS和HDI反应快速固化
已经有11人回复
-
之前让一硕士生水了7个发明专利，现在这7个获批发明专利的维护费可从哪儿支出哈？
已经有11人回复
-
论文投稿求助
已经有4人回复
-
Applied Surface Science 这个期刊。有哪位虫友投过的能把word模板发给我参考一下嘛
已经有3人回复
-
投稿精细化工
已经有6人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- β-糖苷酶表达可溶性蛋白很多，怎么就是没有活性
已经有24人回复
- 求助帮忙下载百度文库课件一个
已经有4人回复
- BL21(DE3)表达脂肪酶重组质粒，将菌体颜色偏白是是怎么回事
已经有17人回复
- 【求助】脂肪酶活力测定
已经有5人回复
- 【求助】脂肪酶
已经有3人回复
- 【求助】酯化反应（可追加奖励）
已经有42人回复
muchong5577
金虫 (正式写手)
- BioEPI: 1
- 应助: 6 (幼儿园)
- 金币: 929.2
- 散金: 67
- 红花: 3
- 帖子: 675
- 在线: 136.6小时
- 虫号: 703384
- 注册: 2009-02-18
- 性别: GG
- 专业: 生物化学
2楼2010-05-10 20:47:11
winter_gates
金虫 (正式写手)
生命过客
- 应助: 1 (幼儿园)
- 金币: 1161.8
- 散金: 100
- 红花: 1
- 帖子: 615
- 在线: 20.4小时
- 虫号: 541629
- 注册: 2008-04-08
- 专业: 生物化学
3楼2010-05-10 21:21:40
winter_gates
金虫 (正式写手)
生命过客
- 应助: 1 (幼儿园)
- 金币: 1161.8
- 散金: 100
- 红花: 1
- 帖子: 615
- 在线: 20.4小时
- 虫号: 541629
- 注册: 2008-04-08
- 专业: 生物化学
4楼2010-05-10 21:22:10
5楼2010-05-10 23:04:18
winter_gates
金虫 (正式写手)
生命过客
- 应助: 1 (幼儿园)
- 金币: 1161.8
- 散金: 100
- 红花: 1
- 帖子: 615
- 在线: 20.4小时
- 虫号: 541629
- 注册: 2008-04-08
- 专业: 生物化学
6楼2010-05-11 10:45:20
7楼2010-05-11 16:53:56
看看这篇文章
muchong5577(金币+1):谢谢~ 2010-05-13 08:43:42
|
J Mol Biol. 2009 Jul 24;390(4):672-85. Epub 2009 May 15. Structure of the alkalohyperthermophilic Archaeoglobus fulgidus lipase contains a unique C-terminal domain essential for long-chain substrate binding. Chen CK, Lee GC, Ko TP, Guo RT, Huang LM, Liu HJ, Ho YF, Shaw JF, Wang AH. Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan. Abstract Several crystal structures of AFL, a novel lipase from the archaeon Archaeoglobus fulgidus, complexed with various ligands, have been determined at about 1.8 A resolution. This enzyme has optimal activity in the temperature range of 70-90 degrees C and pH 10-11. AFL consists of an N-terminal alpha/beta-hydrolase fold domain, a small lid domain, and a C-terminal beta-barrel domain. The N-terminal catalytic domain consists of a 6-stranded beta-sheet flanked by seven alpha-helices, four on one side and three on the other side. The C-terminal lipid binding domain consists of a beta-sheet of 14 strands and a substrate covering motif on top of the highly hydrophobic substrate binding site. The catalytic triad residues (Ser136, Asp163, and His210) and the residues forming the oxyanion hole (Leu31 and Met137) are in positions similar to those of other lipases. Long-chain lipid is located across the two domains in the AFL-substrate complex. Structural comparison of the catalytic domain of AFL with a homologous lipase from Bacillus subtilis reveals an opposite substrate binding orientation in the two enzymes. AFL has a higher preference toward long-chain substrates whose binding site is provided by a hydrophobic tunnel in the C-terminal domain. The unusually large interacting surface area between the two domains may contribute to thermostability of the enzyme. Two amino acids, Asp61 and Lys101, are identified as hinge residues regulating movement of the lid domain. The hydrogen-bonding pattern associated with these two residues is pH dependent, which may account for the optimal enzyme activity at high pH. Further engineering of this novel lipase with high temperature and alkaline stability will find its use in industrial applications. PMID: 19447113 [PubMed - indexed for MEDLINE] |
8楼2010-05-11 16:56:53
winter_gates
金虫 (正式写手)
生命过客
- 应助: 1 (幼儿园)
- 金币: 1161.8
- 散金: 100
- 红花: 1
- 帖子: 615
- 在线: 20.4小时
- 虫号: 541629
- 注册: 2008-04-08
- 专业: 生物化学
9楼2010-05-12 11:36:30
zhangjiewtt
金虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 1381.9
- 散金: 25
- 帖子: 95
- 在线: 49.2小时
- 虫号: 1008919
- 注册: 2010-04-30
- 专业: 微生物遗传育种学
10楼2010-05-12 19:51:20