24小时热门版块排行榜    

查看: 1732  |  回复: 12
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

lnlnln

木虫 (正式写手)

[求助] 冷冻干燥机使用 已有1人参与

由于冻干样品特别少(5mL的瓶,样品是200uL),在冷冻干燥时温度探头该怎么放置好呢?才能精确地监测物料温度?
回复此楼

» 猜你喜欢

» 本主题相关价值贴推荐,对您同样有帮助:

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

lnlnln

木虫 (正式写手)

引用回帖:
7楼: Originally posted by Wuxiroyal at 2016-07-01 15:21:13
sugar is not good, easy boiling, there are lots of other carrier can use, depends on which active compounds  in dry.

If you cannot set temperature, you have to treat the shelf temperature as room ...

The sample belongs to a kind of antigenic proteins, into which sucrose should be added to protect its activity in process of freeze-drying. Whether  we could put ice or ice bag onto the shelf has been assayed and we found the shape after freeze-drying was awful just like sugar boiling.
10楼2016-07-14 16:56:46
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 13 个回答

Wuxiroyal

新虫 (初入文坛)

No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few house or overnight sample will be ready and temperature will never over 4C

Sample 200ul, not sure how much solid residue. you may add bulk agent for example
sugar etc

» 本帖已获得的红花(最新10朵)

2楼2016-06-29 21:44:15
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

lnlnln

木虫 (正式写手)

送红花一朵
引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

Thanks! but I still have a quetion. our equipment can not set temperature, so how to sensor the temperture? and ensure the terminal of dry?
3楼2016-06-30 14:43:40
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

lnlnln

木虫 (正式写手)

引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

these sample contain sucrose, and the concentration is 0.3g/mL。
4楼2016-06-30 14:48:14
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[文学芳草园] 梦想 +5 myrtle 2026-08-26 8/400 2026-09-02 02:42 by Leogzhya
[基金申请] 投票:  有多少人是今天查系统知道结果的? +17 爱看书的可乐 2026-08-26 19/950 2026-09-02 00:19 by xiangy672
[基金申请] 面上合作单位盖章 +6 ssyjh 2026-08-27 9/450 2026-09-01 20:01 by huagongfeihu
[基金申请] 为什么国自然不能直接公布 +5 bjdxyxy 2026-08-26 5/250 2026-09-01 17:54 by 小伟大博士
[论文投稿] 小白求助 投论文要求的highlights应该如何写 5+3 l1963982152 2026-08-29 4/200 2026-09-01 09:04 by 北京莱茵编辑
[基金申请] 国社科又开始会评了,不知道这次命运如何 +7 雨打竹帘 2026-08-30 11/550 2026-08-31 23:16 by hittle2008
[基金申请] 能否申诉? +7 echo8914667 2026-08-30 8/400 2026-08-31 17:00 by yihongxu
[基金申请] 中青基了要发朋友圈吗? +7 349506619 2026-08-28 7/350 2026-08-31 13:39 by 冼亮淀粉酶
[基金申请] 基金不中,共勉 +12 eulota 2026-08-26 12/600 2026-08-31 08:42 by ZJTJZ
[基金申请] 有没有仍没收到信息的 +7 德尚中行 2026-08-27 8/400 2026-08-30 20:52 by purplejack
[考博] 找导师 +6 yuanjiabao 2026-08-29 7/350 2026-08-30 14:40 by 生科新手
[基金申请] 我就是申请一个面上项目而已,这评审意见是按照杰青的条件评的吧? +6 gouxfjh 2026-08-28 11/550 2026-08-30 07:57 by gouxfjh
[基金申请] 国自然面上复盘~欢迎讨论 (金币+15) +15 晴天加油 2026-08-26 16/800 2026-08-29 18:28 by symmetry
[基金申请] 国自然评审意见 +13 wangmingqi 2026-08-28 19/950 2026-08-29 10:22 by Poppy1104
[基金申请] 2026年叶企孙基金 +4 bud_bud 2026-08-27 7/350 2026-08-29 07:23 by foolishmani
[基金申请] 看板上这么多中的,有点像50人群里49个人都是骗子的那种感觉…… +5 a089 2026-08-26 6/300 2026-08-27 14:05 by jonewore
[基金申请] 国际合作可查了,中了面上 (EPI+1)(金币+50) +18 Ldrop2023 2026-08-26 18/900 2026-08-26 11:15 by cmrandy
[基金申请] 系统进不去 +4 yanglien 2026-08-26 5/250 2026-08-26 11:10 by wenfengw83
[基金申请] 国合可查了 +3 paperzjh 2026-08-26 3/150 2026-08-26 10:41 by LemmonTr
[基金申请] 牛来!米来!面来! +8 beefly 2026-08-26 8/400 2026-08-26 08:37 by xuzhipiao
信息提示
请填处理意见