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lnlnln

木虫 (正式写手)

[求助] 冷冻干燥机使用 已有1人参与

由于冻干样品特别少(5mL的瓶,样品是200uL),在冷冻干燥时温度探头该怎么放置好呢?才能精确地监测物料温度?
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Wuxiroyal

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引用回帖:
6楼: Originally posted by lnlnln at 2016-07-01 08:59:09
SCIENTZ-10N,it is awful!...

sugar is not good, easy boiling, there are lots of other carrier can use, depends on which active compounds  in dry.

If you cannot set temperature, you have to treat the shelf temperature as room temperature

If you provide more information about your active compound, I may provide more advice.

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7楼2016-07-01 15:21:13
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Wuxiroyal

新虫 (初入文坛)

No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few house or overnight sample will be ready and temperature will never over 4C

Sample 200ul, not sure how much solid residue. you may add bulk agent for example
sugar etc

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2楼2016-06-29 21:44:15
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lnlnln

木虫 (正式写手)

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引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

Thanks! but I still have a quetion. our equipment can not set temperature, so how to sensor the temperture? and ensure the terminal of dry?
3楼2016-06-30 14:43:40
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lnlnln

木虫 (正式写手)

引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

these sample contain sucrose, and the concentration is 0.3g/mL。
4楼2016-06-30 14:48:14
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