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小木虫论坛-学术科研互动平台 » 生物医药区 » 药品生产 » 工程设备 » 冷冻干燥机使用
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lnlnln

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[求助] 冷冻干燥机使用 已有1人参与

由于冻干样品特别少（5mL的瓶，样品是200uL），在冷冻干燥时温度探头该怎么放置好呢？才能精确地监测物料温度？
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1楼 2016-06-29 16:10:27
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Wuxiroyal

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No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few house or overnight sample will be ready and temperature will never over 4C

Sample 200ul, not sure how much solid residue. you may add bulk agent for example
sugar etc
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2楼2016-06-29 21:44:15
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lnlnln

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引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

Thanks! but I still have a quetion. our equipment can not set temperature, so how to sensor the temperture? and ensure the terminal of dry?
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3楼2016-06-30 14:43:40
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lnlnln

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引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

these sample contain sucrose, and the concentration is 0.3g/mL。
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4楼2016-06-30 14:48:14
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Wuxiroyal

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【答案】应助回帖

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lnlnln: 金币+5, ★★★很有帮助 2016-07-01 08:58:22
引用回帖:
3楼: Originally posted by lnlnln at 2016-06-30 14:43:40
Thanks! but I still have a quetion. our equipment can not set temperature, so how to sensor the temperture? and ensure the terminal of dry?...

tell what type freeze dry ?

you must can set temperature for shelf
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5楼2016-06-30 17:02:38
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lnlnln

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引用回帖:
5楼: Originally posted by Wuxiroyal at 2016-06-30 17:02:38
tell what type freeze dry ?

you must can set temperature for shelf...

SCIENTZ-10N，it is awful！
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6楼2016-07-01 08:59:09
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Wuxiroyal

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引用回帖:
6楼: Originally posted by lnlnln at 2016-07-01 08:59:09
SCIENTZ-10N，it is awful！...

sugar is not good, easy boiling, there are lots of other carrier can use, depends on which active compounds  in dry.

If you cannot set temperature, you have to treat the shelf temperature as room temperature

If you provide more information about your active compound, I may provide more advice.
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7楼2016-07-01 15:21:13
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lnlnln

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The sample belongs to a kind of antigenic proteins, into which sucrose should be added to protect its activity in process of freeze-drying. Whether  we could put ice or ice bag onto the shelf has been assayed and we found the shape after freeze-drying was awful just like sugar boiling.

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8楼2016-07-02 09:37:28
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lnlnln

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7楼: Originally posted by Wuxiroyal at 2016-07-01 15:21:13
sugar is not good, easy boiling, there are lots of other carrier can use, depends on which active compounds  in dry.

If you cannot set temperature, you have to treat the shelf temperature as room ...

thanks!

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9楼2016-07-04 09:57:14
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lnlnln

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引用回帖:
7楼: Originally posted by Wuxiroyal at 2016-07-01 15:21:13
sugar is not good, easy boiling, there are lots of other carrier can use, depends on which active compounds  in dry.

If you cannot set temperature, you have to treat the shelf temperature as room ...

The sample belongs to a kind of antigenic proteins, into which sucrose should be added to protect its activity in process of freeze-drying. Whether  we could put ice or ice bag onto the shelf has been assayed and we found the shape after freeze-drying was awful just like sugar boiling.
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10楼2016-07-14 16:56:46
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