应《网络安全法》要求，自2017年10月1日起，未进行实名认证将不得使用互联网跟帖服务。为保障您的帐号能够正常使用，请尽快对帐号进行手机号验证，感谢您的理解与支持！

24小时热门版块排行榜    

小木虫论坛-学术科研互动平台 » 生物医药区 » 药品生产 » 工程设备 » 冷冻干燥机使用
131/2返回列表12下一页
查看: 1710  |  回复: 12
只看楼主 @他人 存档 新回复提醒 (忽略) 收藏    在APP中查看

lnlnln

木虫 (正式写手)

[求助] 冷冻干燥机使用 已有1人参与

由于冻干样品特别少（5mL的瓶，样品是200uL），在冷冻干燥时温度探头该怎么放置好呢？才能精确地监测物料温度？
回复此楼

» 猜你喜欢

» 本主题相关价值贴推荐，对您同样有帮助:
1楼 2016-06-29 16:10:27
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
回帖支持 ( 显示支持度最高的前 50 名 )

Wuxiroyal

新虫 (初入文坛)
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few house or overnight sample will be ready and temperature will never over 4C

Sample 200ul, not sure how much solid residue. you may add bulk agent for example
sugar etc
赞一下(1人) 回复此楼

» 本帖已获得的红花（最新10朵）

lnlnln
2楼2016-06-29 21:44:15
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
普通回帖

lnlnln

木虫 (正式写手)
送红花一朵
引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

Thanks! but I still have a quetion. our equipment can not set temperature, so how to sensor the temperture? and ensure the terminal of dry?
赞一下 回复此楼
3楼2016-06-30 14:43:40
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

lnlnln

木虫 (正式写手)
引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

these sample contain sucrose, and the concentration is 0.3g/mL。
赞一下 回复此楼
4楼2016-06-30 14:48:14
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

Wuxiroyal

新虫 (初入文坛)

【答案】应助回帖

★ ★ ★ ★ ★
感谢参与，应助指数 +1
lnlnln: 金币+5, ★★★很有帮助 2016-07-01 08:58:22
引用回帖:
3楼: Originally posted by lnlnln at 2016-06-30 14:43:40
Thanks! but I still have a quetion. our equipment can not set temperature, so how to sensor the temperture? and ensure the terminal of dry?...

tell what type freeze dry ?

you must can set temperature for shelf
赞一下 回复此楼
5楼2016-06-30 17:02:38
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

lnlnln

木虫 (正式写手)
引用回帖:
5楼: Originally posted by Wuxiroyal at 2016-06-30 17:02:38
tell what type freeze dry ?

you must can set temperature for shelf...

SCIENTZ-10N，it is awful！
赞一下 回复此楼
6楼2016-07-01 08:59:09
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

Wuxiroyal

新虫 (初入文坛)
引用回帖:
6楼: Originally posted by lnlnln at 2016-07-01 08:59:09
SCIENTZ-10N，it is awful！...

sugar is not good, easy boiling, there are lots of other carrier can use, depends on which active compounds  in dry.

If you cannot set temperature, you have to treat the shelf temperature as room temperature

If you provide more information about your active compound, I may provide more advice.
赞一下 回复此楼

» 本帖已获得的红花（最新10朵）

lnlnln
7楼2016-07-01 15:21:13
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

lnlnln

木虫 (正式写手)
The sample belongs to a kind of antigenic proteins, into which sucrose should be added to protect its activity in process of freeze-drying. Whether  we could put ice or ice bag onto the shelf has been assayed and we found the shape after freeze-drying was awful just like sugar boiling.

发自小木虫Android客户端
赞一下 回复此楼
8楼2016-07-02 09:37:28
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

lnlnln

木虫 (正式写手)
送红花一朵
引用回帖:
7楼: Originally posted by Wuxiroyal at 2016-07-01 15:21:13
sugar is not good, easy boiling, there are lots of other carrier can use, depends on which active compounds  in dry.

If you cannot set temperature, you have to treat the shelf temperature as room ...

thanks!

发自小木虫Android客户端
回复此楼
9楼2016-07-04 09:57:14
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

lnlnln

木虫 (正式写手)
引用回帖:
7楼: Originally posted by Wuxiroyal at 2016-07-01 15:21:13
sugar is not good, easy boiling, there are lots of other carrier can use, depends on which active compounds  in dry.

If you cannot set temperature, you have to treat the shelf temperature as room ...

The sample belongs to a kind of antigenic proteins, into which sucrose should be added to protect its activity in process of freeze-drying. Whether  we could put ice or ice bag onto the shelf has been assayed and we found the shape after freeze-drying was awful just like sugar boiling.
赞一下 回复此楼
10楼2016-07-14 16:56:46
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 lnlnln 的主题更新
131/2返回列表12下一页
普通表情 龙 兔 虎 猫
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考博] 售SCI一区文章，我:8.O.55.1.O.54,科目齐全,可伽急 +3 jCd0dEvKHShX 2026-08-29 5/250 2026-08-30 08:33 by ZPa0EcMwuECS
[考博] 售SCI-T0P文章，我:8O.5.5.1.O.54,科目齐全,可+急 +3 gy1nBQXYQJqL 2026-08-29 5/250 2026-08-30 07:05 by ZPa0EcMwuECS
[找工作] 售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急 +4 gy1nBQXYQJqL 2026-08-29 7/350 2026-08-30 06:46 by ZPa0EcMwuECS
[考研] 售一区SCI文章T0P，我:8O.551.O54,科目全,可十急 +5 ASdOkHsho7FD 2026-08-28 8/400 2026-08-30 06:02 by ZPa0EcMwuECS
[硕博家园] 售SCI一区文章，我:8.O.55.1.O.54,科目齐全,可伽急 +6 ASdOkHsho7FD 2026-08-28 11/550 2026-08-30 06:00 by ZPa0EcMwuECS
[找工作] 售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急 +5 ASdOkHsho7FD 2026-08-28 8/400 2026-08-30 05:51 by ZPa0EcMwuECS
[教师之家] 售SCI-T0P文章，我:8O.5.5.1.O.54,科目齐全,可+急 +5 ASdOkHsho7FD 2026-08-28 8/400 2026-08-30 05:48 by ZPa0EcMwuECS
[论文投稿] 售SCI一区T0P文章，我:8.O55.1.O.54,科目全,可十急 +4 ASdOkHsho7FD 2026-08-28 5/250 2026-08-30 00:41 by ZPa0EcMwuECS
[基金申请] 中青基了要发朋友圈吗？ +4 349506619 2026-08-28 4/200 2026-08-29 22:41 by alongwaytogo
[基金申请] 国自然面上复盘~欢迎讨论 (金币+15) +15 晴天加油 2026-08-26 16/800 2026-08-29 18:28 by symmetry
[基金申请] 麻烦专家们看看评委们的意见（F口面上） +5 gdd2018 2026-08-28 10/500 2026-08-29 14:10 by Jacob678
[基金申请] 基金系统什么内容也没有 30+4 winsaint 2026-08-27 9/450 2026-08-28 11:06 by maolC
[基金申请] 看板上这么多中的，有点像50人群里49个人都是骗子的那种感觉…… +5 a089 2026-08-26 6/300 2026-08-27 14:05 by jonewore
[基金申请] 我不理解！ +15 Edward_pc 2026-08-26 23/1150 2026-08-26 20:34 by zzuzxg
[基金申请] 2026年8月25日国自然放榜前突然收到列入评审专家邮件，有关系吗？ +25 木水思豆 2026-08-25 28/1400 2026-08-26 14:53 by draco1987
[基金申请] 能否退出参与的面上项目解除限项 +23 koalala 2026-08-24 26/1300 2026-08-26 14:29 by 宝贝虫子
[基金申请] 出来了 +9 trojank 2026-08-26 9/450 2026-08-26 14:25 by 宝贝虫子
[基金申请] 国合现在查不到了吗？ +10 chengyan1220 2026-08-24 20/1000 2026-08-26 08:57 by peasantsprig
[基金申请] 明天应该可查了！？ +6 chengyan1220 2026-08-23 6/300 2026-08-25 19:45 by zfd97
[基金申请] 如果此刻你正在为国基感到焦虑，不妨来听听这首《基金之外》 +8 scalable 2026-08-24 8/400 2026-08-25 12:52 by jnhyjjm
信息提示
关闭
请填处理意见
关闭