²é¿´: 1857  |  »Ø¸´: 8

ÜñÂótao

гæ (СÓÐÃûÆø)

[ÇóÖú] RNAÌáÈ¡¹ý³ÌDNA¸ÉÈÅÎÊÌâ-ÓÃÓÚRT-PCR ÒÑÓÐ2È˲ÎÓë

¸÷λºÃ£¬ÎÒÌáÈ¡µÄÖ²ÎïҶƬRNA£¬ÓÃµÄ the Fruit mate for RNA purification (Takara, China)¡£ÌáÈ¡ÍêÒ²½øÐÐÁ˼ì²â£¬ÎÞDNAÔÓÖÊ¡£
µ«ÊÇ·¢ÁËÎÄÕ£¬Éó¸åר¼Ò˵Takara¹«Ë¾ÌáÈ¡µÄRNA±ØÐëÓÃDNAaseI½øÐд¦Àí¡£
ÎÊÌâÊÇÎÒûÓÐÑùÆ·ÁË¡£µ±³õÎÒÒ²½øÐÐÁËDNA¼ì²â£¬Ö¤Ã÷ÁËRNAµÄ´¿¶È¡£Ï£Íû·Ö×ÓÕâ¿éµÄר¼ÒÄÜ´Óרҵˮƽ°ïÎÒÏëÏë¸ÃÈçºÎ½øÐлظ´Éó¸åÒâ¼û£¿£¿
»Ø¸´´ËÂ¥
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

·´Ó¦Á´

гæ (ÕýʽдÊÖ)

How did you detect the DNA contaminants?

In general, it must be set up a negative control, which was omitted reverse transcriptase for DNA contaminant detection.

RNAÌáÈ¡¹ý³ÌDNA¸ÉÈÅÎÊÌâ-ÓÃÓÚRT-PCR
2Â¥2016-01-13 19:16:29
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÜñÂótao

гæ (СÓÐÃûÆø)

ÒýÓûØÌû:
2Â¥: Originally posted by ·´Ó¦Á´ at 2016-01-13 19:16:29
How did you detect the DNA contaminants?

In general, it must be set up a negative control, which was omitted reverse transcriptase for DNA contaminant detection.

...

ÎÒÅܵĵçÓ¾£¬²¢Ó÷ֹâ¹ã¶È½øÐмì²âµÄ¡£ÕâÑù¿ÉÒÔÂð£¿
3Â¥2016-01-13 20:35:46
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

·´Ó¦Á´

гæ (ÕýʽдÊÖ)

ÒýÓûØÌû:
3Â¥: Originally posted by ÜñÂótao at 2016-01-13 20:35:46
ÎÒÅܵĵçÓ¾£¬²¢Ó÷ֹâ¹ã¶È½øÐмì²âµÄ¡£ÕâÑù¿ÉÒÔÂð£¿...

No. Those methods can't detect the extremely small amount of genomic DNA, which could affect the result of RT-PCR.

You must redo RNA preparation with DNaseI treated, otherwise you should submit your paper to the journal with lower IF.

» ±¾ÌûÒÑ»ñµÃµÄºì»¨£¨×îÐÂ10¶ä£©

4Â¥2016-01-14 09:28:07
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÜñÂótao

гæ (СÓÐÃûÆø)

Ëͺ컨һ¶ä
ÒýÓûØÌû:
4Â¥: Originally posted by ·´Ó¦Á´ at 2016-01-14 09:28:07
No. Those methods can't detect the extremely small amount of genomic DNA, which could affect the result of RT-PCR.

You must redo RNA preparation with DNaseI treated, otherwise you should submit  ...

ллÄ㣬ÏëÔÙÎʸöÎÊÌ⣬ÎÒÖªµÀÒýÎïÐòÁУ¬ÈçºÎÖªµÀ»ùÒòÐòÁкţ¿£¿ÎÒÊäÈëÒýÎï²é³öÀ´ºÜ¶à»ùÒò£¬ÉõÖÁÓÚ»ùÒòÃû×Ö¶¼ÊÇÒ»ÑùµÄ£¬¿ÉÊÇÐòÁкŲ»Ò»Ñù¡£ÎÒÔõÃ´Çø·Ö¿ª
5Â¥2016-01-16 20:09:57
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

×ÏÝÇÏãÂý001

гæ (СÓÐÃûÆø)

ÒýÓûØÌû:
2Â¥: Originally posted by ·´Ó¦Á´ at 2016-01-13 19:16:29
How did you detect the DNA contaminants?

In general, it must be set up a negative control, which was omitted reverse transcriptase for DNA contaminant detection.

...

ÇëÎÊ£¬ÎÒÔÚÑéÖ¤DNAÓÐÎÞÎÛȾµÄʱºò£¬¿É²»¿ÉÒÔÕâÑù:кϳɵÄRNA£¬ÓÃÆÕͨPCRµÄº¬TaqøµÄMixÀ´½øÐÐÏà¹Ø»ùÒòµÄÀ©Ôö£¬Èç¹ûÅܽºÖ®ºóÓÐÑôÐÔ½á¹û£¬ÊDz»ÊÇ˵Ã÷ÎÒÌáµÄRNAÀïÃæÓÐDNAÎÛȾ£¿£¿ÕâôÎÊÌâÀ§ÈÅÎÒÁ˺þã¬Ï£ÍûÄúÄÜΪÎÒ½â´ð£¬Ð»Ð»~\(¨R¨Œ¨Q)/~

·¢×ÔСľ³æAndroid¿Í»§¶Ë
6Â¥2016-01-16 21:12:06
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

Ô­À´£¬ÊÇÕâÑù

½ð³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

²»ÄÜÓÃÅܵçÓ¾µÄ·½Ê½ÅжÏÊÇ·ñÓÐÎÞDNA²ÐÁô£¬±ØÐëÒªÓÃDNAøÏû»¯£¬Î¢Á¿µÄDNAÅܽºÊÇ¿´²»µ½µÄ£¬¶øÇÒÓ÷ֹâ¹â¶È¼ÆÊǼì²â²»³öÀ´µÄ£¬¶ÔÄ㷴ת¼֮ºóµÄcDNAÓм¸ÂʲúÉú¸ÉÈÅ£¬ËùÒÔÄǸöÉó¸åµÄµÄÈ·ºÜÑϽ÷£¬ÎÒÃÇÀÏʦҲÊÇÒªÇóÎÒÃDZØÐë×öDNAµÄÏû»¯¡£

·¢×ÔСľ³æAndroid¿Í»§¶Ë
7Â¥2016-01-16 23:20:50
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

xiaoweiwei121

ÈÙÓþ°æÖ÷ (ÎÄ̳¾«Ó¢)

Õâ̶ˮÀ¶µÄÉîåä

ÓÅÐãÇø³¤ÓÅÐãÇø³¤ÓÅÐã°æÖ÷ÓÅÐã°æÖ÷

Õâ¸ö±ØÐëÓÃDNaseI
8Â¥2016-01-17 04:26:19
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÁõÊé½Ü

½ð³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

ÒýÎÉè¼Æ¿çÔ½ÖÁÉÙÒ»¸öÄÚº¬×Ó¡¢¸ù¾Ý²úÎï´óС¾Í¿ÉÒÔÅųýdna¸ÉÈÅ¡£

·¢×ÔСľ³æAndroid¿Í»§¶Ë
9Â¥2016-01-17 06:50:58
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ ÜñÂótao µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼²©] »·¾³ÁìÓòÈ«¹úÖØµãʵÑéÊÒÕÐÊÕ²©Ê¿1-2Ãû +3 QGZDSYS 2026-03-13 3/150 2026-03-18 00:13 by PY-Hzb
[¿¼ÑÐ] 085601ר˶£¬×Ü·Ö342Çóµ÷¼Á£¬µØÇø²»ÏÞ +4 share_joy 2026-03-16 4/200 2026-03-17 21:32 by hmn_wj
[¿¼ÑÐ] 085601²ÄÁϹ¤³Ìר˶Çóµ÷¼Á +5 Ľº®mio 2026-03-16 5/250 2026-03-17 21:31 by hmn_wj
[¿¼ÑÐ] 085601Çóµ÷¼Á +4 Du.11 2026-03-16 4/200 2026-03-17 17:08 by ruiyingmiao
[¿¼ÑÐ] 293Çóµ÷¼Á +6 ÊÀ½çÊ׸» 2026-03-11 6/300 2026-03-17 17:04 by ruiyingmiao
[¿¼ÑÐ] 332Çóµ÷¼Á +6 Zz°æ 2026-03-13 6/300 2026-03-17 17:03 by ruiyingmiao
[¿¼ÑÐ] 08¹¤¿Æ 320×Ü·Ö Çóµ÷¼Á +4 À滨çóÍí·ç 2026-03-17 4/200 2026-03-17 13:38 by houyaoxu
[¿¼ÑÐ] Ò»Ö¾Ô¸»ªÖÐʦ·¶071000£¬325Çóµ÷¼Á +6 RuitingC 2026-03-12 6/300 2026-03-16 14:50 by ¿Éµ­²»¿ÉÍü
[¿¼ÑÐ] 0703 ÎïÀí»¯Ñ§µ÷¼Á +3 ÎÒ¿ÉÒÔÉϰ¶µÄ¶Ô 2026-03-13 5/250 2026-03-16 10:50 by ÎÒ¿ÉÒÔÉϰ¶µÄ¶ÔÂ
[½Ìʦ֮¼Ò] ½¹ÂÇ +7 Ë®±ùÔÂÔÂÒ°Íà 2026-03-13 9/450 2026-03-16 10:00 by Quakerbird
[¿¼²©] ¶«»ªÀí¹¤´óѧ»¯²Äרҵ26½ì˶ʿ²©Ê¿ÉêÇë +6 zlingli 2026-03-13 6/300 2026-03-15 20:00 by ryzcf
[»ù½ðÉêÇë] ÏÖÔÚÈçºÎ»Ø±ÜÈ¥ÄêµÄijһ¸öר¼Ò£¬²»ÖªµÀÃû×Ö +3 zk200107 2026-03-12 6/300 2026-03-14 17:13 by zk200107
[¿¼ÑÐ] 0703£¬333·ÖÇóµ÷¼Á Ò»Ö¾Ô¸Ö£ÖÝ´óѧ-ÎïÀí»¯Ñ§ +3 Àîħٶ·Åñ 2026-03-11 3/150 2026-03-13 22:24 by JourneyLucky
[¿¼ÑÐ] 304Çóµ÷¼Á +7 7712b 2026-03-13 7/350 2026-03-13 21:42 by peike
[¿¼ÑÐ] 310Çóµ÷¼Á +3 ¡¾ÉÏÉÏÇ©¡¿ 2026-03-11 3/150 2026-03-13 16:16 by JourneyLucky
[¿¼ÑÐ] 308Çóµ÷¼Á +3 ÊÇLupa°¡ 2026-03-12 3/150 2026-03-13 14:30 by Çóµ÷¼Ázz
[¿¼ÑÐ] 0817»¯Ñ§¹¤³ÌÓë¼¼Êõ¿¼ÑÐ312·Öµ÷¼Á +3 T123 tt 2026-03-12 3/150 2026-03-13 10:49 by houyaoxu
[¿¼ÑÐ] »¯¹¤Ñ§Ë¶306Çóµ÷¼Á +9 42838695 2026-03-12 9/450 2026-03-13 10:16 by houyaoxu
[¿¼ÑÐ] 081200-11408-276ѧ˶Çóµ÷¼Á +3 ´Þwj 2026-03-12 4/200 2026-03-12 19:33 by Çóµ÷¼Ázz
[¿¼²©] 2026Ä격ʿÉêÇë +3 QwQwQW10 2026-03-11 3/150 2026-03-12 17:58 by gxch43
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û