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How did you detect the DNA contaminants?

In general, it must be set up a negative control, which was omitted reverse transcriptase for DNA contaminant detection.

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2Â¥: Originally posted by ·´Ó¦Á´ at 2016-01-13 19:16:29
How did you detect the DNA contaminants?

In general, it must be set up a negative control, which was omitted reverse transcriptase for DNA contaminant detection.

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3Â¥: Originally posted by ÜñÂótao at 2016-01-13 20:35:46
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No. Those methods can't detect the extremely small amount of genomic DNA, which could affect the result of RT-PCR.

You must redo RNA preparation with DNaseI treated, otherwise you should submit your paper to the journal with lower IF.

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4Â¥: Originally posted by ·´Ó¦Á´ at 2016-01-14 09:28:07
No. Those methods can't detect the extremely small amount of genomic DNA, which could affect the result of RT-PCR.

You must redo RNA preparation with DNaseI treated, otherwise you should submit  ...

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2Â¥: Originally posted by ·´Ó¦Á´ at 2016-01-13 19:16:29
How did you detect the DNA contaminants?

In general, it must be set up a negative control, which was omitted reverse transcriptase for DNA contaminant detection.

...

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