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小木虫论坛-学术科研互动平台» 学术交流区» 论文翻译 » 论文翻译求助完结子版 » 求组文献句子翻译
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袁小小然

银虫 (正式写手)

[求助] 求组文献句子翻译

看文献有一句话不太懂。
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.
这句话怎么翻译好一些呢？
共价结合法倾向于酶特定的方向？？感觉不太对啊！！
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1楼 2015-11-18 23:24:05
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genhunter

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袁小小然: 金币+3 2015-11-19 23:05:24
爱与雨下: 金币+1 2015-11-20 07:09:39
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.

用EDC/NHS方法很到的增幅最少(增加2.8倍), 这可以用共价交联趋于将酶以特定的走向排布来解释。
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离成功还差一点
2楼2015-11-19 14:01:30
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genhunter

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引用回帖:
2楼: Originally posted by genhunter at 2015-11-19 14:01:30
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.

用EDC/NHS方法很到 ...

这里增加的应该是酶的Kd
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离成功还差一点
3楼2015-11-19 14:34:23
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lz3333

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袁小小然: 金币+5 2015-11-19 23:05:37
爱与雨下: 金币+1 2015-11-20 07:09:55
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.
是固定化酶的文献么？我毕业论文做的固定化酶。
翻译需要看上下文的意思，才可以更好的翻译出来，不过就字面的意思应该翻译为：
EDC/NHS法增加的最少，仅2.8倍，共价连接法可以将酶定向（固定化）这一事实可解释这一现象。
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袁小小然
4楼2015-11-19 15:15:20
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袁小小然

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4楼: Originally posted by lz3333 at 2015-11-19 15:15:20
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.
是固定化酶的文献么？ ...

Each sample was a square piece of Whatman no 1 paper with a1 × 1 cm sample area defined with wax (XEROX Phaser printer) [10].Samples for each immobilization method were coded and mea-sured in random order. A 10 L of solution was needed to wet thepaper completely, if not stated otherwise this volume was used forall modifications. All solutions, if not stated differently were pre-pared in PBS pH 5.5. In case of layer-by-layer methods paper wascovered with 3 bilayers starting with anionic polymer. In the secondand third bilayer cationic polymer was mixed with Glucose oxidaseto obtain final concentration of 2.5 mg/mL (92.5 U/mL) of enzyme.In the case of subsequent methods: EDC, EDC/NHS, entrapment inammonium alginate and all encapsulation methods several batcheswith different concentration of reagents and reaction times wereprepared. Table 1 lists only the optimized conditions of aforemen-tioned methods. In case of encapsulation methods, smaller capsuleswere dispersed on sample papers with a pipette, larger capsuleswere transported manually on paper and left to dry.
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5楼2015-11-19 23:03:45
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袁小小然

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引用回帖:
4楼: Originally posted by lz3333 at 2015-11-19 15:15:20
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.
是固定化酶的文献么？ ...

还有一大段帮我看看是什么意思吧！！实在是不知道说的是什么！！
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6楼2015-11-19 23:05:07
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lz3333

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Each sample was a square piece of Whatman no 1 paper with a 1 × 1 cm sample area defined with wax (XEROX Phaser printer) [10].
每个样品都是在whatman 1号方形纸上面的一个1 × 1 cm的样品区域，使用蜡进行固定。
Samples for each immobilization method were coded and measured in random order.
每种固定方法的样品都是随机编号和测量。
A 10 L of solution was needed to wet the paper completely, if not stated otherwise this volume was used for all modifications.
如未标记，所有修饰方法均使用10L溶液来完全润湿样品纸片。
All solutions, if not stated differently were prepared in PBS pH 5.5.
所有溶液，如未特殊标记，均在PBS，pH5.5条件下制备。
In case of layer-by-layer methods paper was covered with 3 bilayers starting with anionic polymer.
层叠法中纸片需用3层膜覆盖，第一层是阴离子聚合物，
In the second and third bilayer cationic polymer was mixed with Glucose oxidase to obtain final concentration of 2.5 mg/mL (92.5 U/mL) of enzyme.
第二层和第三层的阳离子聚合物需要与葡糖氧化酶混合，以达到最终酶浓度为2.5 mg/mL (92.5 U/mL)
你黏贴过来的文献，断句有问题，你看看原文吧，下面是我自己根据意思翻译的。
In the case of subsequent methods: EDC, EDC/NHS, entrapment in ammonium alginate and all encapsulation methods，several batches with different concentration of reagents and reaction times were prepared.
接下来的方法，EDC、 EDC/NHS法中，使用海藻酸铵进行截留；所有封包法中使用不同的试剂浓度和反应时间分别进行了制备。
Table 1 lists only the optimized conditions of a fore-mentioned methods. In case of encapsulation methods, smaller capsules were dispersed on sample papers with a pipette, larger capsules were transported manually on paper and left to dry.
表1仅列举了前面提到方法的最佳条件。在封包法中，小囊使用移液管分散在样品纸上，大囊手动转移到纸上并晾干。
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7楼2015-11-20 09:51:03
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