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袁小小然

银虫 (正式写手)

[求助] 求组文献句子翻译

看文献有一句话不太懂。
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.
这句话怎么翻译好一些呢?
共价结合法倾向于酶特定的方向??感觉不太对啊!!
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lz3333

至尊木虫 (正式写手)

【答案】应助回帖

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袁小小然: 金币+5 2015-11-19 23:05:37
爱与雨下: 金币+1 2015-11-20 07:09:55
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.
是固定化酶的文献么?我毕业论文做的固定化酶。
翻译需要看上下文的意思,才可以更好的翻译出来,不过就字面的意思应该翻译为:
EDC/NHS法增加的最少,仅2.8倍,共价连接法可以将酶定向(固定化)这一事实可解释这一现象。

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4楼2015-11-19 15:15:20
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genhunter

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袁小小然: 金币+3 2015-11-19 23:05:24
爱与雨下: 金币+1 2015-11-20 07:09:39
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.

用EDC/NHS方法很到的增幅最少(增加2.8倍), 这可以用共价交联趋于将酶以特定的走向排布来解释。
离成功还差一点
2楼2015-11-19 14:01:30
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genhunter

至尊木虫 (著名写手)

引用回帖:
2楼: Originally posted by genhunter at 2015-11-19 14:01:30
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.

用EDC/NHS方法很到 ...

这里增加的应该是酶的Kd
离成功还差一点
3楼2015-11-19 14:34:23
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袁小小然

银虫 (正式写手)

引用回帖:
4楼: Originally posted by lz3333 at 2015-11-19 15:15:20
The lowest increase was noted for EDC/NHS method (2.8 times higher),which could be explained by the fact that covalent linkage methods tend to specific orientation of the enzyme.
是固定化酶的文献么? ...

Each sample was a square piece of Whatman no 1 paper with a1 × 1 cm sample area defined with wax (XEROX Phaser printer) [10].Samples for each immobilization method were coded and mea-sured in random order. A 10 L of solution was needed to wet thepaper completely, if not stated otherwise this volume was used forall modifications. All solutions, if not stated differently were pre-pared in PBS pH 5.5. In case of layer-by-layer methods paper wascovered with 3 bilayers starting with anionic polymer. In the secondand third bilayer cationic polymer was mixed with Glucose oxidaseto obtain final concentration of 2.5 mg/mL (92.5 U/mL) of enzyme.In the case of subsequent methods: EDC, EDC/NHS, entrapment inammonium alginate and all encapsulation methods several batcheswith different concentration of reagents and reaction times wereprepared. Table 1 lists only the optimized conditions of aforemen-tioned methods. In case of encapsulation methods, smaller capsuleswere dispersed on sample papers with a pipette, larger capsuleswere transported manually on paper and left to dry.
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5楼2015-11-19 23:03:45
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