| 查看: 206 | 回复: 0 | ||
饿的神啊新虫 (著名写手)
|
[求助]
请懂的战友帮我解释一下（不是要翻译哈）
|
|
基础太差，看文献时不能理解这段话怎么操作的，能不能请懂的战友帮我解释一下（不是要翻译哈） Isolation of gp91phox Homologs from N. benthamiana Two cDNAs for potato rboh, StrbohA and StrbohB, were used as probes to screen an oligo(dT)-primed Uni-ZAP XR library (Stratagene, La Jolla, CA), which was constructed from poly(A)+ RNAs of N. benthamiana leaves treated with hyphal walls of Phytophthora for 6 h. From 6 105 plaques of the library, six positive clones were isolated after three rounds of plaque hybridization under low-stringency conditions by each probe (12 positive clones total). Positive phage plaques were excised directly in vivo into pBluescript SK phagemid according to the manufacturer’s in-structions (Stratagene). The nucleotide sequences were determined for both strands using the Prism Dye Termination Cycle Sequencing Ready Reaction Kit and a DNA sequencing system (model 310; Applied Biosystems, Foster City, CA). The positive clones obtained by the StrbohA probe contained one NbrbohA and five NbrbohB cDNAs, whereas the StrbohB probe detected six NbrbohB cDNAs. The partial sequences of 11 NbrbohB cDNAs were identical. |
回复此楼» 猜你喜欢
推荐一些20种氨基酸检测的实际应用案例
已经有0人回复
-
不合理蛙科研实验中的趣事：实验器材的 “乌龙”
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有283人回复
-
不合理蛙科研实验中的趣事：和实验材料的 “斗智斗勇”
已经有0人回复
-
蛋白质检测：精准分析，解锁生物分子的密码
已经有0人回复
-
不合理蛙科研实验之小鼠实验：严谨设计，解析生命机制的重要载体
已经有0人回复
-
不合理蛙科研实验之重金属检测：精准筛查，守护健康与环境的防线
已经有0人回复
-
不合理蛙科研实验之“蛙测重金属我背锅三千”
已经有0人回复
-
不合理蛙科研实验之“鼠逃三次我发三篇SCI”
已经有0人回复
-
纳米粒度分析
已经有3人回复
10