| 查看: 223 | 回复: 0 | ||
饿的神啊新虫 (著名写手)
|
[求助]
请懂的战友帮我解释一下（不是要翻译哈）
|
|
基础太差，看文献时不能理解这段话怎么操作的，能不能请懂的战友帮我解释一下（不是要翻译哈） Isolation of gp91phox Homologs from N. benthamiana Two cDNAs for potato rboh, StrbohA and StrbohB, were used as probes to screen an oligo(dT)-primed Uni-ZAP XR library (Stratagene, La Jolla, CA), which was constructed from poly(A)+ RNAs of N. benthamiana leaves treated with hyphal walls of Phytophthora for 6 h. From 6 105 plaques of the library, six positive clones were isolated after three rounds of plaque hybridization under low-stringency conditions by each probe (12 positive clones total). Positive phage plaques were excised directly in vivo into pBluescript SK phagemid according to the manufacturer’s in-structions (Stratagene). The nucleotide sequences were determined for both strands using the Prism Dye Termination Cycle Sequencing Ready Reaction Kit and a DNA sequencing system (model 310; Applied Biosystems, Foster City, CA). The positive clones obtained by the StrbohA probe contained one NbrbohA and five NbrbohB cDNAs, whereas the StrbohB probe detected six NbrbohB cDNAs. The partial sequences of 11 NbrbohB cDNAs were identical. |
回复此楼» 猜你喜欢
莫那利珠单抗阻断NKG2A/HLA-E抑制通路 | Biochempartner
已经有0人回复
-
GSPT1口服分子胶降解剂MRT-2359 | Biochempartner
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有120人回复
-
碳青霉烯类抗生素为何需联用西司他丁钠？ | Biochempartner
已经有0人回复
-
源自蕨类植物的抗炎美白活性分子去甲氧基荚果蕨素 | Biochempartner
已经有0人回复
-
肠道里的"代谢开关"：Fexaramine用肠道限制性重新定义 FXR 激动剂
已经有0人回复
-
DMXAA：从血管破坏剂到STING通路研究的关键工具分子 | Biochempartner
已经有0人回复
-
靶向PD-L1的人源化单克隆抗体阿特珠单抗
已经有0人回复
-
"探针之父"(+)-JQ-1如何改变表观遗传研究 | Biochempartner
已经有0人回复
-
含氟糖皮质激素的外用抗炎利器醋酸氟轻松
已经有0人回复
-
阿莫奈韦（Amenamevir）：解旋酶-引物酶靶向分子的结构特征与合成路径全解析
已经有0人回复