²é¿´: 1625  |  »Ø¸´: 9
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

haozy87

½ð³æ (СÓÐÃûÆø)

[ÇóÖú] ÇóÖú£¡µ°°×ÖÊÇеôGST tagºó³ÁÁË ÒÑÓÐ1È˲ÎÓë

ÎÒÔÚBL21Àï±í´ïGST-RASSF1A £¬¹Èë׸ÊëÄÏ´ÍѺó£¬Ã¸ÇÐÇеôGST£¬µ°°×¾Í³ÁÁË£¨µ°°×PI 9.25£©£¬ÊÇbuffer²»¶ÔÂð£¿ÈçºÎɸѡºÏÊʵÄbuffer£¿ÁíÍâGST-RASSF1A¹ÒÔÚbeadsÉÏ£¬²»ÓùÈë׸ÊëÄÏ´ÍÑ£¬Ö±½Ó¼ÓøÇеôGST£¬µ«Ö»eluteÏÂÉÙÁ¿RASSF1A£¬´ó²¿·Ö»¹ÔÚbeadsÉÏ£¬ÈçºÎ·ÀÖ¹µ°°×Çеôtagºó³ÁÔÚbeadsÉÏ£¿Èç¹û³ÁÁË£¬ÔõÑùÏ´ÏÂÀ´£¿Í¬Ñùµ°°×´øHis tag£¬Ò²ÊÇÏàËÆÎÊÌ⣬250mMßäßòֻϴÍÑÁËһС°ëµ°°×£¬´ó²¿·Ö»¹ÔÚNi beadsÉÏ£¬EDTAҲϴ²»ÏÂÀ´£¨ËÀÔÚbeadsÉϵĵ°°×²»×öÏ£Íû£©£¬ÈçºÎ±ÜÃâµ°°×³ÁÔÚNi beadsÉÏ£¿
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

²»Íü³õÐÄ
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

haozy87

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
2Â¥: Originally posted by mlbiosci at 2015-04-19 14:16:53
ÄãµÄbufferµÄph¶àÉÙ£¿µ÷µÄÀëpiÔ¶Ò»µãÊÔÊÔ¡£°´ÕÕbeedsµÄ˵Ã÷Êé²Ù×÷ÎÊÌâÓ¦¸Ã²»´óµÄ¡£ ÄãµÄƽºâbuffer¼ÓµÍŨ¶ÈßäßòÁËÂð£¿ÉÏÑù֮ǰҲÓô˺¬ÓеÍŨ¶ÈßäßòµÄbufferƽºâÖù×ÓÊÔÊÔ¡£

BUffer pH 7.4ºÍ7.0¶¼ÊÔ¹ý£¬Æ½ºâbufferßäßòŨ¶È20mM£¬beads¿Ï¶¨ÊÇÒªÏÈÆ½ºâµÄ¡£
²»Íü³õÐÄ
3Â¥2015-04-19 16:48:11
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 10 ¸ö»Ø´ð

mlbiosci

гæ (ÕýʽдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
ÄãµÄbufferµÄph¶àÉÙ£¿µ÷µÄÀëpiÔ¶Ò»µãÊÔÊÔ¡£°´ÕÕbeedsµÄ˵Ã÷Êé²Ù×÷ÎÊÌâÓ¦¸Ã²»´óµÄ¡£ ÄãµÄƽºâbuffer¼ÓµÍŨ¶ÈßäßòÁËÂð£¿ÉÏÑù֮ǰҲÓô˺¬ÓеÍŨ¶ÈßäßòµÄbufferƽºâÖù×ÓÊÔÊÔ¡£
2Â¥2015-04-19 14:16:53
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

mlbiosci

гæ (ÕýʽдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

ÒýÓûØÌû:
3Â¥: Originally posted by haozy87 at 2015-04-19 16:48:11
BUffer pH 7.4ºÍ7.0¶¼ÊÔ¹ý£¬Æ½ºâbufferßäßòŨ¶È20mM£¬beads¿Ï¶¨ÊÇÒªÏÈÆ½ºâµÄ¡£...

ÎÒ³£ÓõÄÒ²ÊÇÕâ¸öÌõ¼þ£¬³öÏÖ¹ýbeeds¾Û¼¯³ÉÕûÌåµÄÇé¿ö£¬´µÉ¢ºó³Ê´ó¿ÅÁ£×´£¬¸ßŨ¶ÈßäßòÏ´ÍÑҲû¸ÄÉÆ£¬Ê¦ÐÖ˵ÊÇbeeds±»ÆÆ»µÁË

[ ·¢×ÔСľ³æ¿Í»§¶Ë ]
4Â¥2015-04-19 20:36:53
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

haozy87

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
4Â¥: Originally posted by mlbiosci at 2015-04-19 20:36:53
ÎÒ³£ÓõÄÒ²ÊÇÕâ¸öÌõ¼þ£¬³öÏÖ¹ýbeeds¾Û¼¯³ÉÕûÌåµÄÇé¿ö£¬´µÉ¢ºó³Ê´ó¿ÅÁ£×´£¬¸ßŨ¶ÈßäßòÏ´ÍÑҲû¸ÄÉÆ£¬Ê¦ÐÖ˵ÊÇbeeds±»ÆÆ»µÁË
...

Beads ȷʵ²»ÐÐÁË£¬ÎÊÌâÊÇÐÂbeadsЧ¹ûҲûÌáÉý¶àÉÙ¡£ÏÖÔÚÖ»ÄÜÔÙ»»ÔØÌ壬»»±í´ïϵͳÁË
²»Íü³õÐÄ
5Â¥2015-04-20 12:16:44
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] ²ÄÁÏ¿ÆÑ§Ó빤³Ì 317Çóµ÷¼Á +4 JKSOIID 2026-03-26 4/200 2026-03-26 15:58 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] 292Çóµ÷¼Á +8 ¶ì¶ì¶ì¶î¶î¶î¶î¶ 2026-03-25 9/450 2026-03-26 12:21 by ÕÅ¿­Ê®°ËºÅ
[¿¼ÑÐ] Çóµ÷¼Á +3 «lty 2026-03-25 4/200 2026-03-25 23:25 by «lty
[¿¼ÑÐ] ÇóbÇøÔºÐ£µ÷¼Á +4 ÖÜ56 2026-03-24 5/250 2026-03-25 17:12 by yishunmin
[¿¼ÑÐ] 359Çóµ÷¼Á +3 ÍõÁ˸öéª 2026-03-25 3/150 2026-03-25 12:50 by Dyhoer
[¿¼ÑÐ] 285Çóµ÷¼Á +3 AZMK 2026-03-24 3/150 2026-03-25 12:23 by userper
[¿¼ÑÐ] Ò»Ö¾Ô¸ÎäÀí085500»úеרҵ×Ü·Ö300Çóµ÷¼Á +3 an10101 2026-03-24 7/350 2026-03-25 00:00 by ɽ¹í0-
[¿¼ÑÐ] ʳƷר˶ һ־Ը˫һÁ÷ 328 +3 xiaom99 2026-03-21 4/200 2026-03-24 21:20 by lailaisimei
[¿¼ÑÐ] ²ÄÁÏ¿¼Ñе÷¼ÁÉú +3 »ÆÁ»Ò»ÃÎǧÄê 2026-03-24 3/150 2026-03-24 17:00 by barlinike
[¿¼ÑÐ] Ò»Ö¾Ô¸ÄϺ½²ÄÁÏר317·ÖÇóµ÷¼Á +5 ըѽըѽըÊíÌõ 2026-03-23 5/250 2026-03-24 16:52 by ÐÇ¿ÕÐÇÔÂ
[¿¼ÑÐ] 292Çóµ÷¼Á +4 ¶ì¶ì¶ì¶î¶î¶î¶î¶ 2026-03-24 4/200 2026-03-24 16:41 by peike
[¿¼ÑÐ] Çóµ÷¼ÁÒ»Ö¾Ô¸Î人Àí¹¤´óѧ²ÄÁϹ¤³Ì£¨085601£© +5 WW.' 2026-03-23 7/350 2026-03-24 14:50 by sprinining
[¿¼ÑÐ] 305·ÖÇóµ÷¼Á£¨Ê³Æ·¹¤³Ì£© +5 Sxy112 2026-03-21 7/350 2026-03-24 12:27 by 544594351
[¿¼ÑÐ] 269Çóµ÷¼Á +4 ÎÒÏë¶ÁÑÐ11 2026-03-23 4/200 2026-03-23 21:25 by pswait
[¿¼ÑÐ] 333Çóµ÷¼Á +3 ALULU4408 2026-03-23 3/150 2026-03-23 19:04 by macy2011
[¿¼ÑÐ] 275Çóµ÷¼Á +6 shansx 2026-03-22 8/400 2026-03-22 15:27 by barlinike
[¿¼ÑÐ] 269ר˶Çóµ÷¼Á +6 ½ð¶÷±´ 2026-03-21 6/300 2026-03-22 14:31 by ColorlessPI
[¿¼ÑÐ] ¿¼Ñе÷¼Á +3 ºôºô£¿~+123456 2026-03-21 3/150 2026-03-21 20:04 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 0805²ÄÁÏ320Çóµ÷¼Á +3 ÉÎïÓï 2026-03-20 3/150 2026-03-21 15:46 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 353Çóµ÷¼Á +3 À­¹³²»Ðí±ä 2026-03-20 3/150 2026-03-20 19:56 by JourneyLucky
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û