Prior to immobilization of the aptamers, the biotinylated aptamer solution (14 μM aptamers, dissolved in 50 mM Tris-HCl + 20 mM KCl + 600 mM NaCl, pH 7.4) was heated at 90 °C for 3 min followed by cooling at room temperature. The heat treatment was a common practice applied to allow the aptamers to form the desired conformation through renaturation.
请问：以上文献将适配体90 °C加热3分钟后，不会失活了吗？还能继续用来对特定蛋白质的萃取吗？
回复此楼
