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In this study, we used a two-step PCR protocol as previously described (19). With this approach, tags and adapters were added in a second round of PCR amplification. This method facilitates DNA amplification from complex biological samples (20), increases reproducibility, and recovers higher genetic diversity, avoiding the amplification bias that is introduced by using long fusion primers in one-step PCR amplification¡£ ÎÒÊÇС°×Ò»¸ö£¬ÕâÀïÃæµÄtag ºÍadapter·Ö±ðÊÇʲôÒâ˼°¡£¿»¹ÓУ¬×îÖ÷ÒªµÄ:Õâ¸ötwo-step PCR ÊÇÒ»¸öʲô·½·¨£¬Äܲ»Äܾ;ßÌ彲һϣ¬ÒòΪҪÏòÀÏʦ»ã±¨ÕâÆªÎÄÕ£¬µ«ÊǶÔÕâ¸öÍêÈ«²»Á˽Ⱑ£¬È¥ÄÄÀïÕÒÕÒÕâ·½ÃæµÄ×ÊÁÏ£¿ |
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