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zbr405新虫 (初入文坛)
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[求助]
审稿意见求助 已有2人参与
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各位虫友,我投的是Sensors and Actuators B, 17天就返回了审稿意见，大家帮帮忙，帮我理解一下审稿人的意思是什么，审稿意见如下： Reviewers' comments: Reviewer #1: In this paper, the author prepared electrochemiluminescence aptasensing sensor based on ferrocene-graphene nanosheets for the detection of thrombin. However, the following issues need to be addressed before accept for publication: (1) The English of the manuscript needs improvement. (2) In the paper, the author said the ECL intensity of the Ru(bpy)32+ was quenched by Fc, but through the paper, the author did not prove the ECL intensity was actually quenched by Fc or graphene, as graphene also is a quencher. (3) In the experimental section, the author needs to briefly state how Fc-GNs were synthesized. (4) In fig. 6, what the author mean ECL intensity vs. time curves? Why not use the traditional ECL intensity vs. potential curves? (5) Statistical dada are missing in the figs with error bars, how many tests are repeated? Reviewer #2: This manuscript is a report containing innovation with new data for detection of thrombin. It is worthy to be published after some minor revisions. Some suggestions are listed as followings: 1. The voltage supplied to the photomuliplier for luminescence intensity determination needs to be confirmed. 2. A brief description about the mechanism of ECL through TPrA is better to be included in the manuscript. 3. A possible explanation for the optimal pH to give best activity is suggested. 4. The Nyquist plot was used to demonstrate a increase in charge transfer resistance after the formation of the insulating Ru-TBA layer. For a insulating layer, the electron resistance should also increase and showed an increase in the intercept with the Z' axis at high frequency. The manuscript is better to show this behavior. 5. Figure 5 shows that the ECL intensity change, <DELTA>IECL, was about 100 for Fc-GNs/Ru-TBA modified GCE. But, the ECL intensity change was about 300 without thrombin according calibration curve in Figure 6. Is there any explanation for this difference? |
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F_Shaw
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2楼2014-06-18 11:04:52
carefu
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【答案】应助回帖
★ ★ ★
感谢参与，应助指数 +1
zbr405: 金币+3, 谢谢 2014-06-18 14:52:48
感谢参与，应助指数 +1
zbr405: 金币+3, 谢谢 2014-06-18 14:52:48
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我尝试理解一下吧， 审稿人1： 1、英语太烂了，得改。 2、你直接提出来一种解释，却没有实验来证实。（这个，如果实验实在做不出来，可以说由于实验条件限制什么的，然后找几篇相关的文献引证一下就行） 3、简要说明制备方法。 4.为什么要用密度时间曲线？ 5、这个应该是说你的统计曲线里，只有误差棒，但是把原来的实验数据给隐藏了。还有，要额外点明统计了多少次实验的数据。 审稿2 ： 1、好像是测定光强吧，应该是补数据的意思 2、简述机制 3、解释活性最佳ph值。 4、审稿人提出来一种什么现象或解释之类的，我是外行，不懂，楼主自己理解吧。 5、Fig5和6不符，要求给个解释。 感觉都不难回答，也没有特别犀利的问题。希望很大的 |
3楼2014-06-18 11:21:09
