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swingsinsky

[½»Á÷] digest Ö®ºódnaÏûʧ£¬ÕÒ²»µ½Ô­ÒòÁË£¬ÇóÖú

after double digesting with Bamh and NdeI all my dna was gone
1. BSA(10) 3ul, buffer 3 3ul, dH2O 12 ul, DNA 5ul, Bamh 1ul, NdeI 1ul
2. BSA(10) 3ul, buffer 2 3ul, dH2O 12 ul, DNA 5ul, Bamh 1ul, NdeI 1ul
3. BSA(10) 3ul, buffer 3 3ul, dH2O 12 ul, DNA 5ul
4. BSA(10) 1ul, buffer 6 1ul, dH2O 6.5 ul, DNA 2ul, Bamh 0.5ul, NdeI 0.5ul

37C, 1h

after running the gel, I can only find band in 3, which is the control

it's really hard for me to figure it out, because I already changed the amount in 4, and changed different buffers,
and I reduced the digestion time to 1h, so what happened to my DNA? I really need help here. Many thanks!!

plus, I added the BSA buffer and ddwater first, DNA second, and added the enzyme at last
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wangjun0457

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2Â¥2008-02-29 10:50:27
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swingsinsky

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3Â¥2008-02-29 11:07:15
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wangjiasi

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hejiayang

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swingsinsky

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6Â¥2008-02-29 13:19:19
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sofu

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7Â¥2008-02-29 13:25:10
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sofu

½ð³æ (ÕýʽдÊÖ)

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swingsinsky(½ð±Ò+1,VIP+0):thanks
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"An expert is a man who has made all the mistakes which can be made, in a very narrow field." -- Niels Henrik David Bohr
8Â¥2008-02-29 13:27:15
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swingsinsky

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