| 查看: 822 | 回复: 16 | |||
| 当前主题已经存档。 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[交流]
digest 之后dna消失，找不到原因了，求助
|
|||
|
after double digesting with Bamh and NdeI all my dna was gone 1. BSA(10) 3ul, buffer 3 3ul, dH2O 12 ul, DNA 5ul, Bamh 1ul, NdeI 1ul 2. BSA(10) 3ul, buffer 2 3ul, dH2O 12 ul, DNA 5ul, Bamh 1ul, NdeI 1ul 3. BSA(10) 3ul, buffer 3 3ul, dH2O 12 ul, DNA 5ul 4. BSA(10) 1ul, buffer 6 1ul, dH2O 6.5 ul, DNA 2ul, Bamh 0.5ul, NdeI 0.5ul 37C, 1h after running the gel, I can only find band in 3, which is the control it's really hard for me to figure it out, because I already changed the amount in 4, and changed different buffers, and I reduced the digestion time to 1h, so what happened to my DNA? I really need help here. Many thanks!! plus, I added the BSA buffer and ddwater first, DNA second, and added the enzyme at last |
回复此楼» 猜你喜欢
一志愿郑大材料工程290求调剂
已经有6人回复
-
292求调剂
已经有11人回复
-
309求调剂
已经有11人回复
-
262求调剂
已经有3人回复
-
303求调剂
已经有5人回复
-
300求调剂，材料科学英一数二
已经有17人回复
-
一志愿北京化工大学材料与化工（085600）296求调剂
已经有21人回复
-
307求调剂
已经有15人回复
-
286分调剂
已经有9人回复
-
求调剂323材料与化工
已经有8人回复
5楼2008-02-29 12:19:50
2楼2008-02-29 10:50:27
3楼2008-02-29 11:07:15
wangjiasi
木虫 (正式写手)
- 应助: 5 (幼儿园)
- 金币: 2382.9
- 散金: 120
- 红花: 4
- 帖子: 346
- 在线: 737.9小时
- 虫号: 450308
- 注册: 2007-11-04
- 性别: GG
- 专业: 生化分析及生物传感
4楼2008-02-29 12:08:39
