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digest 之后dna消失，找不到原因了，求助
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after double digesting with Bamh and NdeI all my dna was gone 1. BSA(10) 3ul, buffer 3 3ul, dH2O 12 ul, DNA 5ul, Bamh 1ul, NdeI 1ul 2. BSA(10) 3ul, buffer 2 3ul, dH2O 12 ul, DNA 5ul, Bamh 1ul, NdeI 1ul 3. BSA(10) 3ul, buffer 3 3ul, dH2O 12 ul, DNA 5ul 4. BSA(10) 1ul, buffer 6 1ul, dH2O 6.5 ul, DNA 2ul, Bamh 0.5ul, NdeI 0.5ul 37C, 1h after running the gel, I can only find band in 3, which is the control it's really hard for me to figure it out, because I already changed the amount in 4, and changed different buffers, and I reduced the digestion time to 1h, so what happened to my DNA? I really need help here. Many thanks!! plus, I added the BSA buffer and ddwater first, DNA second, and added the enzyme at last |
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