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pET-28a(+) sequence landmarks
T7 promoter 370-386
T7 transcription start 369
His•Tag coding sequence 270-287
T7•Tag coding sequence 207-239
Multiple cloning sites
(BamH I - XhoI) 158-203
His•Tag coding sequence 140-157
T7 terminator 26-72
lacIcoding sequence 773-1852
pBR322 origin 3286
Kan coding sequence 3995-4807
f1 origin 4903-5358
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