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[求助] 怎么测蛋白质巯基含量

求助各位,肉类蛋白质巯基含量的测定,看了文献都是用DTNB法测定,可是各种步骤搞不清楚。比如说,The final thiol concentration was calculated on the basis of afive-point standard curve ranging from 5 to 200μM thiols prepared fromL-cysteine diluted in 5.0% SDS/Tris buffer (pH 8.0). The thiol concentrations in nanomoles thiol per milligram protein are presented as the mean±SD of three independent replicates.这一段是怎么弄的,求教!~~
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