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mengyouren

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1¡¢ The problem faced by the xylanases using in pulp and paper industry,  however,  is  retaining  active  under  the  extreme  process  condition  of  high  temperature  and  high  alkali.
2¡¢All the positive mutants were detected by their ability to produce a hydrolysis zone on xylan plates (LB-Agar plate added 1% xylan, 100 ¦Ìg/mL ampicillin, and 1mM IPTG, pH 7.0), then were transfered to alkali xylan plates which at pH 10.0 for the products of the first ground epPCR and pH 11.0 for the products of the second ground epPCR.
3¡¢Collected  the clones revealing relative higher xylanase activity compared with that of E. coli BL21 (pET-XynG1-1) for further enzymatic properties studying and gene sequencing.
4¡¢ The  results  in  Table  4  showed  that  both  XynG1-1  and  XynG1-1B43cc  pretreatment  indicated distinct effect in the bleaching of cotton stalk pulp.
5¡¢XynG1-1 and XynG1-1B43cc pretreatment gained  brightness  levels  of  73.39%  and  78.95%  which revealed  obvious  higher  than that  (69.66%)  of  control (Table 4).
6¡¢All of the results indicated that the mutant xylanase  XynG1-1B43cc is more available to the high temperature and high alkali process condition of paper industry.
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stingdhk

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hanches: ½ð±Ò+1, ¸ÐлӦÖú£¬BB½±Àø~~~ 2013-11-07 09:40:43
mengyouren: ½ð±Ò+10 2013-11-08 11:21:48
The problem faced by the xylanases ¸öÈËÈÏΪ¸ÄΪThe problem that the xylanases faced ±È½ÏºÃ
3Â¥2013-11-06 15:23:26
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befair

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¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ...
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hanches: ½ð±Ò+3, ¸ÐлÈÈÐÄÓ¦Öú£¬BB½±Àø~~~~ 2013-11-05 21:24:16
mengyouren: ½ð±Ò+90, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸ 2013-11-08 11:21:39
1¡¢ The problem faced by the xylanases used in pulp and paper industry,  however,  is  retaining  active  under  the  extreme  process  condition  of  high  temperature  and  high  alkali.
2¡¢All the positive mutants were detected by their ability to produce a hydrolysis zone on xylan plates (LB-Agar plate added 1% xylan, 100 ¦Ìg/mL ampicillin, and 1mM IPTG, pH 7.0), and then to be transferred to alkali xylan plates, at pH 10.0 for the products of the first ground epPCR and pH 11.0 for those of the second.
×¢£ºÕâÀïÎÒÀí½â to produce ºÍ to be transferred²¢ÁУ¬¹²Í¬À´ÐÞÊÎability¡£Èç¹ûÂß¼­²»¶Ô, transferred ...ÊǺÍwere detected ...²¢ÁеÄ,ÄÇôÕâ¾ä»°¾ÍÓ¦¸Ã¸Ä³É£º
All the positive mutants were detected by their ability to produce a hydrolysis zone on xylan plates (LB-Agar plate added 1% xylan, 100 ¦Ìg/mL ampicillin, and 1mM IPTG, pH 7.0), and were then transferred to alkali xylan plates, at pH 10.0 for the products of the first ground epPCR and pH 11.0 for those of the second.
3¡¢The collected clones revealed relatively higher xylanase activity compared with that of E. coli BL21 (pET-XynG1-1), which requires further enzymatic property study and gene sequencing.
×¢£ºÕâÀïǰ°ë¾äºÍºó±ßµÄËÆºõûʲôÁ¬½Ó£¬ÕâÀï¼ÙÉèǰ°ë¾ä˵µÄÇé¿öÐèÒªurther enzymatic property study and gene sequencing£¬Èç¹û²»Êǵϰ£¬ÇëÖ¸³öÈ·ÇÐÂß¼­¹ØÏµ
4¡¢ The  results  in  Table  4  showed  that  both  XynG1-1  and  XynG1-1B43cc  pretreatment  had distinct effects on bleaching of cotton stalk pulp.
5¡¢By XynG1-1 and XynG1-1B43cc pretreatment brightness  levels  of  73.39%  and  78.95% were obtained,    which  are obviously  higher  than that  (69.66%)  of  the control  one (Table 4).
6¡¢All the results indicated that the mutant xylanase  XynG1-1B43cc is more tolerant to the high temperature and high alkali process condition of paper industry than others¡£
×¢£º´ÓÉÏÎÄÀí½âmutant xylanase  XynG1-1B43cc¶ÔÓÚhigh temperature and high alkali process condition of paper industryÓ¦¸ÃÊDZȽϲ»Ãô¸Ð¡¢²»´óÊÜÓ°ÏìµÄ£¬ËùÒÔÓÃÁËtolerant¡£Ô­ÒëÎÄis more availableÒÉËÆÏë˵mutant xylanase  XynG1-1B43cc¶ÔÓÚ¸ßΡ£¡£¡£¸üºÃÓ㬵«available²»Íס£ÁíÍâÓñȽϼ¶µ«Ã»Óб»±È½Ï¶ÔÏó¡£ÎÒÓÃÁËthan others,Æäʵ²»¹»È·¶¨£¬»¹ÇëÂ¥Ö÷Õå×õ½µ×ÏëºÍʲô±È£¬ È»ºó´úÌæ"others"
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Befairorbeaware
2Â¥2013-11-05 21:18:43
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