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宜初xx

铜虫 (初入文坛)

应助: 0 (幼儿园)
金币: 44.8
帖子: 8
在线: 7.5小时
虫号: 2070798
注册: 2012-10-18
专业: 基因表达调控与表观遗传学

[求助] cufflinks问题求助

植物转录组高通量测序数据，做差异表达分析，用tophat-cufflinks，cufflinks运行完成后得到的transcripts.gtf为0，报错如下：

SAM error on line 33727900: CIGAR op has zero length
SAM error on line 33782251: CIGAR op has zero length
SAM error on line 33834635: CIGAR op has zero length
SAM error on line 33858553: CIGAR op has zero length
SAM error on line 33880254: CIGAR op has zero length
SAM error on line 33890249: CIGAR op has zero length
SAM error on line 33908272: CIGAR op has zero length
SAM error on line 33917229: CIGAR op has zero length
SAM error on line 33928144: CIGAR op has zero length
SAM error on line 33943859: CIGAR op has zero length
SAM error on line 33957629: CIGAR op has zero length
SAM error on line 33972718: CIGAR op has zero length
SAM error on line 33996263: CIGAR op has zero length
SAM error on line 34005315: CIGAR op has zero length
SAM error on line 34035650: CIGAR op has zero length
SAM error on line 34049621: CIGAR op has zero length
SAM error on line 34122824: CIGAR op has zero length
SAM error on line 34124971: CIGAR op has zero length
SAM error on line 34127014: CIGAR op has zero length
SAM error on line 34127475: CIGAR op has zero length
SAM error on line 34178143: CIGAR op has zero length
SAM error on line 34186043: CIGAR op has zero length
SAM error on line 34210002: CIGAR op has zero length
SAM error on line 34258453: CIGAR op has zero length
SAM error on line 34268289: CIGAR op has zero length
SAM error on line 34307456: CIGAR op has zero length
SAM error on line 34332244: CIGAR op has zero length
SAM error on line 34352836: CIGAR op has zero length
SAM error on line 34388695: CIGAR op has zero length
SAM error on line 34424573: CIGAR op has zero length
SAM error on line 34435716: CIGAR op has zero length
SAM error on line 34441799: CIGAR op has zero length
SAM error on line 34459558: CIGAR op has zero length
SAM error on line 34467635: CIGAR op has zero length
SAM error on line 34635079: CIGAR op has zero length
SAM error on line 34781892: CIGAR op has zero length
> Processed 0 loci. [*************************] 100%

哪位高手帮忙给看看吧？谢谢

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