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gyj0428

新虫 (初入文坛)

[求助] 玻璃片抗菌操作

大家好,小妹在玻璃片上接了抗菌蛋白,之后抗菌,按如下操作步骤,每次空白玻璃都不长细菌,怎么回事,先谢了。
Airborne  bacteria test:
Collected E.coli DH5 α cells were diluted to 1.0 × 10 7 /mL. To imitate the
aerosolized, airborne bacteria, the cells suspension in sterile saline solution was
sprayed onto glass coverslip coated with SiNWs@AgNPs nanostructure (2.0 × 2.0cm), dried in a fume hood for 5 min 26 . Autoclaved LB growth medium (with 1.5% agars) that was pre-cooled to 40 ℃ was added to the bacteria-exposed cover glasses. Once the agar solidified, the dish was incubated at 37 ℃ overnight.
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北程

新虫 (初入文坛)

红舟流星: 欢迎新虫来生物材料板块交流,有问题可以开新帖询问 2013-08-28 23:14:23
我的也不长,请问你的问题解决了吗??那个雾化怎么实现的啊??用什么喷雾??
2楼2013-08-28 11:28:02
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