²é¿´: 2445  |  »Ø¸´: 33
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

luba

гæ (СÓÐÃûÆø)


[½»Á÷] ¿Ë¡ÇóÖú£¬¶¼Á½¸ö¶àÔÂÁË

Òª×öÒ»¸ö¿Ë¡£¬°ÑÒѾ­¹¹½¨ºÃµÄÒ»¸öÔØÌå(pCDNA4)ÉϵÄÒ»¸ö»ùÒòÇÐÏÂÀ´£¬»»³ÉÁíÒ»¸ö¡£¶øÕâÁíÒ»¸ö»ùÒòÒ²ÊÇ´ÓÒ»¸öÔØÌå(pCDNA3.1)ÉÏÇÐÏÂÀ´µÄ¡£Óõ½µÄ˫øÇÐλµãÊÇBamHIºÍSpeI,¶¼ÊÇÓõÄTAKARAµÄ£¬10*Kbuffer,37¶È¡£Ö®Ç°ÒòΪpCDNAϵÁÐÔØÌåÉ϶¼ÓÐÁ½¸öSpeIλµã£¬ÇÐÏÂÀ´ºÃ¶àÌõ´ø£¬¾Í°ÑpCDNA4×öÁ˸ö¶¨µãÍ»±ä£¬È»ºóÔÙÇУ¬¾ÍÖ»ÓÐÎÒÒªµÄÌõ´øÁË¡£ËùÒԸоõÇÐÓ¦¸ÃûÓÐÎÊÌ⣬ÒòΪÇÐÏÂÀ´µÄÌõ´ø¶¼ÄÜ¿´µ½¡£Á¬½ÓÒ²ÊǹýÒ¹µÄ¡£¾ÍÊDz»³¤¿Ë¡£¬»¹×öÁ˵¥µ¥ÔØÌåµÄ¶ÔÕÕ£¬Ò²Ã»³¤¡£Ò»¸ö¾ú¶¼Ã»ÓУ¡¾ÍÓÐÒ»´Î£¬ÄôíÁË£¬»¹ÄÃÁËÔ­À´Ã»×ö¹ýÍ»±äµÄpCDNA4À´Çе쬽á¹û»¹³¤ÁË£¬ÌôÁ˼¸¸ö¿Ë¡£¬ÓÃÁíÒ»¸öø¼ø¶¨ÁËÏ£¬Ã²ËÆÊÇÁ¬ÔÚÁíÒ»¶ÔB-SµÄλÖÃÉÏÁË£¨Ç°ÃæËµ¹ý£¬ÓÐÁ½¸öSpeIλµã£¬BamHIλµãÔÚÕâÁ½¸öÖм䣩£¬²»¹ýΪÁ˱£ÏÕÒ²ÄÃÈ¥²âÐòÁË£¬»¹²»ÖªµÀ½á¹û¡£¡£¡£¡£
»¹×ö¹ý·Ö²½Ã¸ÇУ¬×ª»¯Ê±Ò²°ÑÁ¿¼Ó´óÁË£¬¸ÐÊÜ̬ÓùýDH5A£¬Ò²ÓùýJM109,ÕæÊÇʲô·½·¨¶¼ÓùýÁË£¬ÊµÔÚ²»ÏþµÃÔ­Òò£¡ºÜ·³£¡Ê²Ã´ÊÂÇ鶼ûÐÄÇéÁË£¡Çë´ó¼Ò°ïÎÒÏëÏ룬³ö³öÖ÷Ò⣡ÔÙÕâÑùÏÂÈ¥ÎÒÒ²²»ºÃÒâ˼ÔÙ´ýÕßÁË£¬Ì«´ò»÷ÈËÁË¡£¡£¡£¡£
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

» ÇÀ½ð±ÒÀ²£¡»ØÌû¾Í¿ÉÒԵõ½:

²é¿´È«²¿É¢½ðÌù

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

luba

гæ (СÓÐÃûÆø)


ÒýÓûØÌû:
12Â¥: Originally posted by ÃÎæÃæÃÓñÀö at 2012-08-28 11:18:39
Â¥Ö÷¼ÓÓÍ£¬°ô°ôµÄ

лл£¡
21Â¥2012-08-28 21:26:03
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 34 ¸ö»Ø´ð

yandz680717

ľ³æ (ÕýʽдÊÖ)


¡ï ¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
1949stone: ½ð±Ò+1, ¹ÄÀø 2012-08-28 07:20:50
»»Á¬½Óø£¬×¢ÒâÔØÌåºÍÍâԴƬ¶ÎµÄĦ¶û±È1:5-1:10
3Â¥2012-08-28 06:13:17
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

lidonghong

½ð³æ (ÕýʽдÊÖ)


¡ï ¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
Сµ¤Ä¾Ä¾: ½ð±Ò+1, ¹ÄÀø½»Á÷ 2012-08-30 14:36:51
Ê×ÏÈÄãÒª±£Ö¤ÔØÌå(pCDNA4)ºÍÄ¿µÄ»ùÒòøÇгɹ¦£¬È»ºóÔÙ°´ÕÕÂ¥ÉϵıÈÀý½øÐÐÁ¬½Ó£¬Á¬½ÓʱµÄÌõ¼þ¿ÉÒÔÐÞ¸ÄÏ£¬²»Ò»¶¨Òª16¶È¹ýÒ¹£¬18¶È 22¶ÈÁ¬½ÓÒ²ÊÇ¿ÉÒԵ쬾ßÌåÌõ¼þ¿´¿´ÄãµÄÁ¬½ÓøʹÓÃ˵Ã÷Ê飡
4Â¥2012-08-28 08:36:00
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

snzydong

Ìú¸Ëľ³æ (ÖøÃûдÊÖ)


¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
Ö÷ÒªÎÊÌâÊÇÄãÁ¬½Ó²»³¤ ×öת»¯µÄʱºòÓжÔÕÕô ¶ÔÕÕ³¤ÁËô ¸ÐÊÜ̬ÊÇÂòµÄô ÓÐÆ·Öʱ£Ö¤Ã´
5Â¥2012-08-28 08:50:35
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] ѧ˶274Çóµ÷¼Á +3 LiÀîÓã 2026-03-26 3/150 2026-03-26 18:32 by Ìá³ö·½·¨µÄÌá³öº
[¿¼ÑÐ] 321Çóµ÷¼Á +5 wasdssaa 2026-03-26 5/250 2026-03-26 18:00 by fmesaito
[¿¼ÑÐ] Ò»Ö¾Ô¸»ªÀí£¬ÊýÒ»Ó¢Ò»285ÇóAÇøµ÷¼Á +4 AZMK 2026-03-25 6/300 2026-03-26 16:12 by ÍõСǷi
[¿¼ÑÐ] ±±¿Æ281ѧ˶²ÄÁÏÇóµ÷¼Á +17 tcxiaoxx 2026-03-20 19/950 2026-03-26 16:04 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] ²ÄÁÏ¿ÆÑ§Ó빤³Ì 317Çóµ÷¼Á +4 JKSOIID 2026-03-26 4/200 2026-03-26 15:58 by ²»³Ôô~µÄ؈
[²ÄÁϹ¤³Ì] Ò»Ö¾Ô¸C9²ÄÁÏÓ뻯¹¤×¨Òµ×Ü·Ö300Çóµ÷¼Á +5 Âü111 2026-03-24 6/300 2026-03-26 13:04 by 13756423260
[¿¼ÑÐ] 303Çóµ÷¼Á +6 À¶É½Ô 2026-03-25 6/300 2026-03-25 22:47 by 418490947
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤328·Öµ÷¼Á +6 ¡££¬¡££¬¡££¬¡£i 2026-03-23 6/300 2026-03-25 22:30 by 418490947
[¿¼ÑÐ] ²ÄÁÏÇóµ÷¼Á +4 .m.. 2026-03-25 4/200 2026-03-25 21:30 by peike
[¿¼ÑÐ] 296Çóµ÷¼Á +4 Íô£¡£¿£¡ 2026-03-25 7/350 2026-03-25 16:41 by Íô£¡£¿£¡
[¿¼ÑÐ] ²ÄÁÏѧ˶333Çóµ÷¼Á +4 ±±µÀÏï 2026-03-24 4/200 2026-03-25 14:16 by mapenggao
[¿¼ÑÐ] 289²ÄÁÏÓ뻯¹¤£¨085600£©BÇøÇóµ÷¼Á +4 ÕâôÃû×ÖÕ¦Ñù 2026-03-22 5/250 2026-03-25 08:20 by mx.yue
[¿¼ÑÐ] ²ÄÁÏר˶ÕÒµ÷¼Á +5 ¹þ¹þ¹þºðºðºð¹þ 2026-03-23 5/250 2026-03-24 19:07 by ÁËÁËÁËÁË¡£¡£
[¿¼ÑÐ] 292Çóµ÷¼Á +4 ¶ì¶ì¶ì¶î¶î¶î¶î¶ 2026-03-24 4/200 2026-03-24 16:41 by peike
[¿¼ÑÐ] 361Çóµ÷¼Á +3 Glack 2026-03-22 3/150 2026-03-23 22:03 by fuyu_
[¿¼ÑÐ] ÉúÎïѧһ־Ը985£¬·ÖÊý349Çóµ÷¼Á +6 zxts12 2026-03-21 9/450 2026-03-23 18:37 by macy2011
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤085600£¬×Ü·Ö304£¬±¾¿ÆÓÐÁ½Æªsci²ÎÓ룬Çóµ÷¼Á +4 ÐÒÔ˵Ľ´½´ 2026-03-22 5/250 2026-03-22 20:15 by edmund7
[¿¼ÑÐ] Çóµ÷¼ÁÒ»Ö¾Ô¸º£´ó£¬0703»¯Ñ§Ñ§Ë¶304·Ö£¬Óдó´´ÏîÄ¿£¬Ëļ¶Òѹý +6 ÐÒÔËÁ¨Á¨ 2026-03-22 10/500 2026-03-22 20:10 by edmund7
[¿¼ÑÐ] Çóµ÷¼ÁԺУÐÅÏ¢ +6 CX 330 2026-03-21 6/300 2026-03-22 15:25 by ÎÞи¿É»÷111
[¿¼ÑÐ] Ò»Ö¾Ô¸ÄÏ´ó£¬0703»¯Ñ§£¬·ÖÊý336£¬Çóµ÷¼Á +3 ÊÕµ½VS 2026-03-21 3/150 2026-03-21 18:42 by ѧԱ8dgXkO
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û