The iTC200 system is the most sensitive isothermal titration calorimeter available. It is designed to address the needs of today’s life science researchers – particularly those engaged in drug discovery and development. With its reduced size and associated sample quantity requirements, the iTC200 is significantly faster than previous models. Designed for ease-of-use, all functions of the iTC200 are operated through software to facilitate fast and accurate analyses.
The iTC200 utilizes a 200 μL cell versus the 1.4 mL cell in previous models.
Applications include:
Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.
Lead optimization.
Enzyme kinetics.
Assessment of the effect of molecular structure changes on binding mechanisms.
Assessment of biological activitiy.
Why iTC200?
More than just affinities: Simultaneous determination of all binding parameters in a single experiment - information unobtainable from more limited binding assays.
Directly measure sub-millimolar to nanomolar binding constants (102 to 109 M-1). Measure nanomolar to picomolar binding constants (109 to 1012 M-1) using the competitive binding technique.
Application versatility: Investigate any biomolecular interaction with high sensitivity. The sample cell is 200 μL. As little as 10 μg of protein can be used.
True in-solution technique: No labeling or immobilization required. No buffer restrictions. Easily handles turbid solutions.
Easy to use: Includes user friendly experimental design wizards and easy filling and cleaning procedures.
Fast time to first result: With fast equilibration times, up to two runs per hour can be easily accomplished. Requires no assay development or dedicated user to achieve high quality results.
Automatable: iTC200 can be upgraded to a fully automated version with a sample throughput of at least 75 samples per day with a capacity to process as many as 384 samples unattended.
At Rest
Sample Loading
Titration
Washing
iTC200 features:
Active cell volume ~200 μL.
Non-reactive Hastelloy® cells for excellent chemical resistance.
Fixed-in-place cells for reproducible ultrasensitive performance with low maintenance.
Precision liquid delivery system for accurate and reproducible injections
User-selectable mixing speeds to match sample conditions.
Three user selectable response times (U.S. Patent number 5,967,659) for application versatility.
Operating temperature range of 2oC to 80oC.
Peltier controlled for rapid temperature equilibration.
Includes the iTC200 Washing Module for easy cell and syringe filling and cleaning.
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