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¶îÃôµÄÑ©: ½ð±Ò+5 2013-01-29 10:53:05
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Plasma membrane vesicles were isolated from Arabidopsis leaves using two-phase partitioning according to a procedure described previously (Qiu et al., 2002). The membrane vesicles were resuspended in a 50 mM Tris-HCl buffer, pH 7.5, and used immediately for NADPH oxidase activity assays. A total of 1 ¦ÌM 16:0-18:2 PA, 16:0-18:2 PC, or lysoPA or 10 ¦ÌM (¡À) ABA (final ethanol, 0.02% [v/v]) was applied to the membrane vesicles (3 to 6 ¦Ìg), respectively, and incubated in the reaction buffer (50 mM Tris-HCl buffer, pH 7.5, and 0.5 mm XTT). NADPH (50 ¦ÌM) was used to initiate the reaction. After 10 min at 25¡ãC, the reaction solution was used for spectrophotometric analysis of XTT formazan production at A470. NADPH activity was expressed as ¦¤A470 per milligram protein per minute. ¦¤A470 represents the difference in XTT formazan absorbance at 470 nm in the presence and absence of 100 units of SOD.

Zhang et al. The Plant Cell August 2009 vol. 21 no. 8 2357-2377

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4Â¥: Originally posted by starseacow at 2013-01-28 19:07:46
ÎÒÒÔǰ²âÖ²ÎïNADPHÑõ»¯Ã¸»îÐÔ·½·¨ÈçÏÂ
Plasma membrane vesicles were isolated from Arabidopsis leaves using two-phase partitioning according to a procedure described previously (Qiu et al., 2002). The  ...

ÄãºÃ£¬ÎÒÒ²Òª²âÖ²ÎïҶƬÖÐNADPHÑõ»¯Ã¸»îÐÔ£¬Ã»Ã÷°×Õâ¾ä»°ÊÇʲôÒâ˼£¬ÄãÄܲ»ÄÜ×Ðϸ½²Ò»½²°¡£¡ A total of 1 ¦ÌM 16:0-18:2 PA, 16:0-18:2 PC, or lysoPA or 10 ¦ÌM (¡À) ABA (final ethanol, 0.02% ) was applied to the membrane vesicles (3 to 6 ¦Ìg), respectively, and incubated in the reaction buffer (50 mM Tris-HCl buffer, pH 7.5, and 0.5 mm XTT).
5Â¥2015-07-11 17:02:40
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