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tingspdf

至尊木虫 (著名写手)

[求助] 定量PCR扩增效率过高(SYBR)

向各位朋友求助一下,最近做一个基因的定量,前面有三步法,标线斜率-2.63,后来改为两步法,标线稍有改善,但还是-2.83,远远没有达到90%-110%的要求,求助一下这种情况该怎么优化条件?
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tingspdf

至尊木虫 (著名写手)

顶起来

[ 发自手机版 http://muchong.com/3g ]
2楼2012-05-24 14:07:41
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tingspdf

至尊木虫 (著名写手)

顶起来
3楼2012-05-25 09:02:58
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孙启亮

金虫 (著名写手)

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与,应助指数 +1
tingspdf: 金币+5, ★★★很有帮助, 好的,我试试。非常感谢! 2012-05-25 09:41:26
tingspdf: 金币+5, ★★★很有帮助 2012-05-31 10:40:56
做个NTC,看是不是有非特异性扩增,扩增效率过高通常不是因为条件的原因,很可能有非特异性扩增
4楼2012-05-25 09:14:11
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