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castlered

木虫 (小有名气)

应助: 9 (幼儿园)
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虫号: 460224
注册: 2007-11-15
性别: MM
专业: 病原细菌与放线菌生物学

[求助] 求助ncbi序列提交问题

前几天提交了几个细菌的基因簇序列，收到如下回信：
We have noticed that some or all of the sequences contain internal stop
>codons and/or reading frame shifts (insertions/deletions based on BLAST
>similarity search results and/or an alignment).

This generally indicates problems such as:
>
>  - errors in the nucleotide sequence
>  - problems with feature annotation which may be due to:
> translation in the wrong reading frame or on the wrong strand
> translation does not have the correct exon spans
> translation includes noncoding sequence, such as introns or UTR's
>  - sequence may be a pseudogene
>
>If the sequences and/or annotation are incorrect, please resubmit
>with the corrected files. If the information is correct, please explain
>the presence of the internal stop codons and/or frame shifts.
>
>If you are unable to determine the correct sequence and/or annotation
>for your sequence(s), they can still be processed and the following
>comment will be added to your records:
>
>COMMENT  GenBank staff is unable to verify sequence and/or annotation
>       provided by the submitter.
>
>In addition, the record(s) will indicate that the data are unverified and
>your sequence(s) will not be included in NCBI BLAST databases.
>
>Please either:
>
> [1] resubmit with correct sequence(s) and/or annotation
> or
> [2] confirm that your sequence(s) should be processed as unverified.
我的序列都是用artemis进行的预测，ncbi上blast注释的，应该没有内部的终止密码子出现的，移码突变这个倒是有可能出现，但我一共提交了10个序列，每个都十几个orf呢，怎么快速检查解决这种状况呢？非常感谢

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