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[求助]
膜蛋白提取试剂盒问题
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| 最近要做真菌膜蛋白的分离提取~我想问一下膜蛋白提取试剂盒能行吗？ 试剂盒原理是什么丫，一次提取的量有多少，可以提取多少次？ 谢谢各位前辈了 |
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xiaoyu0127
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3楼2013-06-28 10:18:04
xhjggl
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【答案】应助回帖
感谢参与，应助指数 +1
绿茶珍(金币+5): 2012-03-01 08:36:00
绿茶珍(金币+5): 2012-03-01 08:36:00
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FOCUS™ Global Fractionation 膜蛋白分级分离和胞质蛋白提取试剂盒 Description: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins. Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localized proteins have been shown to be involved in intracellular membrane trafficking and signaling. where one prep is:100mg mammalian tissue or 50µl wet animal cell pellet or 50µl wet yeast pellet or 50µl wet bacteria pellet or 250mg plant tissue. Store：-15～-20℃ Features: Fractionate integral, peripheral and lipid raft associated proteins.Fractionation of complex proteomes into multiple, specific fractions.applification for Selective fractionation of membrane proteins from tissues, cells, plants, yeast, bacteria, insects, and other sources.Fractionation of membrane proteins from lipid rafts.Suitable for membrane protein preparation for 2D gel electrophoresis and other applications.Suitable for proteomics and cell signaling studies. 编号 包装 价格 产地 786-018 50 Assays 2406 G Bios |
2楼2012-02-29 22:43:29