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忘了补充图片中的一些注释了:Functional complementation of S. cerevisiae ctr1Dctr3D mutant (MPY17) by coexpression of Xa13, COPT1, and COPT5. Complementation is indicated by growth on the media with 0 or 5 mM Cu. The p413, p414, and p416 are yeast expression vectors. Yeast ScCtr1 and empty vector (V) were used as positive and negative controls, respectively. Transformants were grown in SC-His-Trp-Ura medium to exponential phase and spotted onto SC-His-Trp-Ura, ethanol/glycerol (YPEG), and bathocuproine disulfonic acid (BCS) plates. |
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