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2楼2011-09-05 09:54:53

lxj341401

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【答案】应助回帖

3楼2011-09-05 09:55:24

labrat

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★ ★ ★ ★ ★
dhd997(金币+5, MolEPI+1): good 2011-09-05 18:46:04
baiyal2000(金币+2): 2011-09-09 10:50:31

Abstract—Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction
endonucleases, nicking endonucleases cleave only one predetermined DNA strand. Until recently, nicking endonucleases were suggested to be naturally mutated restriction endonucleases which had lost their ability to dimerize and as a result the ability to cleave the second strand.

Recognition Site:
5'...CCTCAGC...3'
3'...GGAGT(cut site)CG...5'

Ref: http://www.protein.bio.msu.su/bi ... 74/pdf/bcm_1457.pdf

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4楼2011-09-05 13:22:50

moqing1991

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引用回帖:

1070663楼: Originally posted by labrat at 2011-09-05 13:22:50
Abstract—Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition ...

我想问下，这个酶会不会受重金属离子影响？

我想做个木头人

5楼2012-09-11 11:02:20

guwenshu2013

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引用回帖:

5楼: Originally posted by moqing1991 at 2012-09-11 11:02:20
我想问下，这个酶会不会受重金属离子影响？...

不受重金属影响。

家总要成，钱总要挣，奔走红尘，莫忘曾经是书生

6楼2013-10-25 23:01:04

141320209

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引用回帖:

4楼: Originally posted by labrat at 2011-09-05 13:22:50
Abstract—Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition ...

亲，我想请教下，这个酶要哪里买得到。还有识别位点有那些呀？是不是要完整的识别位点存在才会剪切呢？（比如说..CCTCAGC...和.GGAGT(cut site)CG这个只有完整的时候才切割还是存在TCG就会切割呢？）我是新手。再次感谢！

7楼2016-03-17 22:55:04

一笑作春温23

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引用回帖:

7楼: Originally posted by 141320209 at 2016-03-17 22:55:04
亲，我想请教下，这个酶要哪里买得到。还有识别位点有那些呀？是不是要完整的识别位点存在才会剪切呢？（比如说..CCTCAGC...和.GGAGT(cut site)CG这个只有完整的时候才切割还是存在TCG就会切割呢？）我是新手。再次 ...

请问这个问题现在解决了吗？我也遇到同样的问题了~谢谢！

8楼2018-05-29 18:49:45