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【答案】应助回帖
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dhd997(金币+5, MolEPI+1): good 2011-09-05 18:46:04
baiyal2000(金币+2): 2011-09-09 10:50:31
Abstract—Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction
endonucleases, nicking endonucleases cleave only one predetermined DNA strand. Until recently, nicking endonucleases were suggested to be naturally mutated restriction endonucleases which had lost their ability to dimerize and as a result the ability to cleave the second strand.
Recognition Site:
5'...CCTCAGC...3'
3'...GGAGT(cut site)CG...5'
Ref: http://www.protein.bio.msu.su/bi ... 74/pdf/bcm_1457.pdf |
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