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本帖产生 2 个 MolEPI ，点击这里进行查看

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lxj341401

至尊木虫 (著名写手)

MolEPI: 58
应助: 388 (硕士)
贵宾: 0.238
金币: 17138.7
红花: 43
帖子: 2251
在线: 712.4小时
虫号: 111074
注册: 2005-11-20
专业: 生物化学

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dhd997(金币+5, MolEPI+1): good 2011-09-05 18:45:51
baiyal2000(金币+1): 2011-09-09 10:50:26

Description:
Nt.BbvCI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate.
Source:
An E. coli strain expressing an altered form of the BbvCI restriction genes [Ra(K169E):Rb+] from Bacillus brevis (L. Ge).
识别位点、作用方式等见以下链接：
http://www.neb.com/nebecomm/products/productR0632.asp

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2楼2011-09-05 09:54:53

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

labrat

木虫 (著名写手)

MolEPI: 3
应助: 5 (幼儿园)
金币: 2250.2
帖子: 2297
在线: 13.1小时
虫号: 100291
注册: 2005-11-12
性别: GG
专业: 生物化学

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dhd997(金币+5, MolEPI+1): good 2011-09-05 18:46:04
baiyal2000(金币+2): 2011-09-09 10:50:31

Abstract—Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction
endonucleases, nicking endonucleases cleave only one predetermined DNA strand. Until recently, nicking endonucleases were suggested to be naturally mutated restriction endonucleases which had lost their ability to dimerize and as a result the ability to cleave the second strand.

Recognition Site:
5'...CCTCAGC...3'
3'...GGAGT(cut site)CG...5'

Ref: http://www.protein.bio.msu.su/bi ... 74/pdf/bcm_1457.pdf

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4楼2011-09-05 13:22:50

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