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小木虫论坛-学术科研互动平台 » 生物医药区 » 分子生物 » 综合其他 » the nicking enzyme Nb. BbvC I 是什么酶？
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1楼 2011-09-05 09:09:13
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moqing1991

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1070663楼: Originally posted by labrat at 2011-09-05 13:22:50
Abstract—Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition  ...

我想问下，这个酶会不会受重金属离子影响？
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我想做个木头人
5楼2012-09-11 11:02:20
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lxj341401

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dhd997(金币+5, MolEPI+1): good 2011-09-05 18:45:51
baiyal2000(金币+1): 2011-09-09 10:50:26
Description:
Nt.BbvCI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate.
Source:
An E. coli strain expressing an altered form of the BbvCI restriction genes [Ra(K169E):Rb+] from Bacillus brevis (L. Ge).
识别位点、作用方式等见以下链接：
http://www.neb.com/nebecomm/products/productR0632.asp
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2楼2011-09-05 09:54:53
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lxj341401

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Description:
Nt.BbvCI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate.
Source:
An E. coli strain expressing an altered form of the BbvCI restriction genes [Ra(K169E):Rb+] from Bacillus brevis (L. Ge).
识别位点、作用方式等见以下链接：
http://www.neb.com/nebecomm/products/productR0632.asp
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3楼2011-09-05 09:55:24
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labrat

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dhd997(金币+5, MolEPI+1): good 2011-09-05 18:46:04
baiyal2000(金币+2): 2011-09-09 10:50:31
Abstract—Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction
endonucleases, nicking endonucleases cleave only one predetermined DNA strand. Until recently, nicking endonucleases were suggested to be naturally mutated restriction endonucleases which had lost their ability to dimerize and as a result the ability to cleave the second strand.

Recognition Site:
5'...CCTCAGC...3'
3'...GGAGT(cut site)CG...5'

Ref: http://www.protein.bio.msu.su/bi ... 74/pdf/bcm_1457.pdf
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4楼2011-09-05 13:22:50
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