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7Â¥: Originally posted by 141320209 at 2016-03-17 22:55:04
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8Â¥2018-05-29 18:49:45
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lxj341401

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dhd997(½ð±Ò+5, MolEPI+1): good 2011-09-05 18:45:51
baiyal2000(½ð±Ò+1): 2011-09-09 10:50:26
Description:
Nt.BbvCI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate.
Source:
An E. coli strain expressing an altered form of the BbvCI restriction genes [Ra(K169E):Rb+] from Bacillus brevis (L. Ge).
ʶ±ðλµã¡¢×÷Ó÷½Ê½µÈ¼ûÒÔÏÂÁ´½Ó£º
http://www.neb.com/nebecomm/products/productR0632.asp
2Â¥2011-09-05 09:54:53
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lxj341401

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

Description:
Nt.BbvCI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate.
Source:
An E. coli strain expressing an altered form of the BbvCI restriction genes [Ra(K169E):Rb+] from Bacillus brevis (L. Ge).
ʶ±ðλµã¡¢×÷Ó÷½Ê½µÈ¼ûÒÔÏÂÁ´½Ó£º
http://www.neb.com/nebecomm/products/productR0632.asp
3Â¥2011-09-05 09:55:24
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

labrat

ľ³æ (ÖøÃûдÊÖ)

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¡ï ¡ï ¡ï ¡ï ¡ï
dhd997(½ð±Ò+5, MolEPI+1): good 2011-09-05 18:46:04
baiyal2000(½ð±Ò+2): 2011-09-09 10:50:31
Abstract¡ªNicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction
endonucleases, nicking endonucleases cleave only one predetermined DNA strand. Until recently, nicking endonucleases were suggested to be naturally mutated restriction endonucleases which had lost their ability to dimerize and as a result the ability to cleave the second strand.

Recognition Site:
5'...CCTCAGC...3'
3'...GGAGT(cut site)CG...5'

Ref: http://www.protein.bio.msu.su/bi ... 74/pdf/bcm_1457.pdf
4Â¥2011-09-05 13:22:50
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