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qwky2010

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Originally posted by lxj341401 at 2011-06-24 19:20:20:
Double Digestion with EcoRI, NotI

We recommend:
Buffer O
EcoRI
NotI
Incubate at 37¡ãC

Other options (presented in the order of decreasing restriction enzyme activity):
Buffer EcoRI
E ...

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qwky2010(½ð±Ò+5): лл°ïÎҲ鰡£¬ºÇºÇ 2011-06-24 19:28:26
amisking(EPI+1): 2011-06-24 20:15:12
ÒýÓûØÌû:
Originally posted by qwky2010 at 2011-06-24 19:08:20:
ÏÖÔÚ²»ÄÜÔÚ¸ÃÍøÕ¾²éѯÁË£¬ÖªµÀµÄ³æÓÑÄÜ·ñ¸æËßϰ¡£¬Ð»Ð»ÁË

Double Digestion with EcoRI, NotI

We recommend:
Buffer O
EcoRI
NotI
Incubate at 37¡ãC

Other options (presented in the order of decreasing restriction enzyme activity):
Buffer EcoRI
EcoRI
2-fold excess of NotI
Incubate at 37¡ãC

Buffer 2X Tango™
EcoRI
4-fold excess of NotI
Incubate at 37¡ãC
2Â¥2011-06-24 19:20:20
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lxj341401

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

ÒýÓûØÌû:
Originally posted by qwky2010 at 2011-06-24 19:49:47:
Âé·³Äã¸æËßÎÒһϣ¬Õâ¸ö˫øÇÐÌåϵÖУ¬Ã¸µÄ¼ÓÈëÁ¿ÊǼ¸±È¼¸¹ØÏµÄØ£¬EcoRI£º NotI-1:1£¬»¹ÊÇ1:2£¬»òÊÇ2:1°¡£¬Ð»Ð»ÁË

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» ±¾ÌûÒÑ»ñµÃµÄºì»¨£¨×îÐÂ10¶ä£©

4Â¥2011-06-24 19:58:44
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qwky2010

½ð³æ (ÖøÃûдÊÖ)

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ÒýÓûØÌû:
Originally posted by lxj341401 at 2011-06-24 19:58:44:
Ñ¡ÔñÍÆ¼öµÄ»º³åÒºÊÇ1:1£¬µÚ¶þ¸ö»º³åÒºÊÇ2:1£¬µÚÈý¸ö»º³åÒºÊÇ4:1¡£Ó¢Óï˵µÄºÜÃ÷°×µÄ£¡

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