±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 3170  |  »Ø¸´: 24
±¾Ìû²úÉú 1 ¸ö BioEPI £¬µã»÷ÕâÀï½øÐв鿴

beautybanana

ľ³æ (ÖøÃûдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

Table2. pMD18-T vector ligation system
pMD18-T                        1uL
DNA template        4uL
Solution I                        5uL
Total                        10uL

16¡æ±£ÎÂ3¡«4hºó£¬È«Á¿£¨10uL£©×ª»¯ÖÁ150uLµÄE.coli DH5¦ÁµÄ¸ÐÊÜ̬ϸ°û¡£
ÉÏÃæÊÇÎÒÒÔǰ×öÁ´½Óת»¯µÄ·½·¨£¬»¹ÓиÐÊÜ̬ϸ°ûÒª±£Ö¤ÄÜÓ㬶à×ö¼¸¹Ü£¬Ò²Ðí¾ÍÓоú°ßÁË¡£
11Â¥2011-05-10 14:31:37
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yabxxh

ľ³æ (ÕýʽдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï
silicare(½ð±Ò+2): лл²ÎÓ룬Á¬½ÓøµÄÖÖÀàÓкöàÖÖ~ 2011-05-10 15:26:23
ºÇºÇ£¬²»ÖªµÀÄãÓõÄÄļҵÄÁ¬½Óø£¬Ôõô»áÓÐÕâÑùµÄζȺÍʱ¼ä¡£
    Ê×ÏÈÀ´½²Î¶ȵϰ£¬³ý·ÇÄã×öÑǿˡ£¬»òÕßÄãÆ¬¶ÎGCº¬Á¿¹ý¸ß£¬¶¼ÊÇ16¶È×îºÃ£¬¶øÇÒ×îºÃÓÃPCR¿ØÎ¡£µ±È»ÊÔ¼ÁºÐÓÃ22Ò²ÊÇÓÐËû×Ô¼ºµÄÄ¿µÄµÄ¡£
    Æä´Î£¬ÄãµÄƬ¶ÎÏà¶ÔÓÚÔØÌåÒѾ­ºÜ´óÁË£¬ÄãÂòµÄT4²»ÊÇ¿ìËٵذѣ¬Æäʵ¿ìËÙÁ¬½ÓøÔÚÁ¬½ÓСƬ¶ÎµÄʱºò»¹±È½ÏÓÐÓÅÊÆ£¬µ«ÊÇ´óÆ¬¶ÎµÄÔØÌå¹¹½¨£¬²¢ÎÞÈκÎÓÅÊÆ¿ÉÑÔ¡£
´óƬ¶ÎµÄÔØÌå¹¹½¨»¹ÊÇÒªÀÏÀÏʵʵµÄ16¶È¹ýÒ¹Á¬½Ó£¬ÕâÑù²ÅÄܱ£Ö¤Á¬½ÓЧÂÊ£¬ÒòΪ´ó·Ö×Ó¼äµÄÁ¬½ÓÇ£Éæµ½¿Õ¼ä¹¹ÏóµÄÎÊÌâ¡£
    »¹ÓÐÒ»¸öºÜÖØÒªµÄÊÇÄãµÄÔØÌåºÍÄ¿µÄƬ¶ÎµÄĦ¶û±È£¬Õâ¸öÓо­ÑéµÄÈË¿ÉÒÔ¿¼ÈâÑÛÀ´Æ¬¶ÎÁ½ÕßÓ¦¼ÓµÄÌå»ý±È£¬Ã»Óо­ÑéµÄ¾ÍÀÏÀÏʵʵµÄÈ¥²â¸öOD°É£¬Êµ¼ùÖ¤Ã÷²âOD»¹ÊǺÜÓÐЧµÄ¡£
×îºóÄãµÄÄ¿µÄƬ¶ÎµÄŨ¶ÈÒ»¶¨Òª¹»¸ß£¬Ì«µÍÁ˲»Ðеġ£
×££¬ºÃÔË
12Â¥2011-05-10 14:41:27
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yoyoyoyo3985

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
Originally posted by beautybanana at 2011-05-10 14:31:37:
Table2. pMD18-T vector ligation system
pMD18-T                        1uL
DNA template        4uL
Solution I                        5uL
Total                        10uL

16¡æ±£ÎÂ3¡«4hºó£¬È«Á¿£¨10uL£©×ª»¯ÖÁ150uLµÄE.coli ...

·Ç³£¸Ðл
ÄÑÃâÂñԹʱ¼äµÄÊÖ£¬°ÑÏమд³ÉÏà°®¹ý
13Â¥2011-05-10 16:06:59
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yoyoyoyo3985

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
Originally posted by yabxxh at 2011-05-10 14:41:27:
ºÇºÇ£¬²»ÖªµÀÄãÓõÄÄļҵÄÁ¬½Óø£¬Ôõô»áÓÐÕâÑùµÄζȺÍʱ¼ä¡£
    Ê×ÏÈÀ´½²Î¶ȵϰ£¬³ý·ÇÄã×öÑǿˡ£¬»òÕßÄãÆ¬¶ÎGCº¬Á¿¹ý¸ß£¬¶¼ÊÇ16¶È×îºÃ£¬¶øÇÒ×îºÃÓÃPCR¿ØÎ¡£µ±È»ÊÔ¼ÁºÐÓÃ22Ò²ÊÇÓÐËû×Ô¼ºµÄÄ¿µÄµÄ¡£
    Æä ...

àÅàÅ,ѧϰÁË,·Ç³£¸Ðл
ÄÑÃâÂñԹʱ¼äµÄÊÖ£¬°ÑÏమд³ÉÏà°®¹ý
14Â¥2011-05-10 16:07:32
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zhangxn2010

ľ³æ (ÖøÃûдÊÖ)

ÈÃÒ»ÇÐËæ·ç·ÉÏè

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï
lstt09nk(½ð±Ò+3): ¹ÄÀøÏ 2011-05-11 18:43:56
ÕâÊÇǰ¶Îʱ¼äÒ»Ö±À§ÈÅÎÒµÄÎÊÌ⣬²»¹ýÏÖÔÚ½â¾öÁË¡£
Ê×ÏÈ£¬ÄãµÃ±£Ö¤Á¬½ÓÌåϵÖи÷Ƭ¶ÎDNAµÄÖÊÁ¿£¨°üÀ¨Å¨¶ÈºÍ´¿¶È£©£¬Õâ¸öºÜÖØÒª£¬µ±³õÎÒ¾ÍÊÇÒòΪʹÓÃÁËTBEÇнº£¬¶à×öÁËÁ½¸öÔÂʵÑé¶¼²»³ö½á¹û£¨¹úÄڵĽº»ØÊÕÊÔ¼ÁºÐ×îºÃÓÃTAE×ö»º³åÒº£©¡£
Á¬½Óǰ¿ÉÒÔ42¶ÈÈÈ»÷¼¸·ÖÖÓÔÙ¼ÓÁ¬½Óø£¬16¡ãC¹ýÒ¹¡£
ÏÂÃæ¾ÍÊÇת»¯ÁË£¬Ò»¶¨Òª×ö¶ÔÕÕ£¨ÑôÐÔandÒõÐÔ£©£¬ÕâÑù²ÅÄܱ£Ö¤ÄãµÄ¸÷ÖÖÌõ¼þûÎÊÌâ¡£
×ÜÖ®£¬ºÃÔ˰ɣ¡
Ò»Ö±Ïòǰ£¡
15Â¥2011-05-10 16:07:46
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yoyoyoyo3985

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
Originally posted by yabxxh at 2011-05-10 14:41:27:
ºÇºÇ£¬²»ÖªµÀÄãÓõÄÄļҵÄÁ¬½Óø£¬Ôõô»áÓÐÕâÑùµÄζȺÍʱ¼ä¡£
    Ê×ÏÈÀ´½²Î¶ȵϰ£¬³ý·ÇÄã×öÑǿˡ£¬»òÕßÄãÆ¬¶ÎGCº¬Á¿¹ý¸ß£¬¶¼ÊÇ16¶È×îºÃ£¬¶øÇÒ×îºÃÓÃPCR¿ØÎ¡£µ±È»ÊÔ¼ÁºÐÓÃ22Ò²ÊÇÓÐËû×Ô¼ºµÄÄ¿µÄµÄ¡£
    Æä ...

ÎÒÏÖÔÚµÄÄ¿µÄ»ùÒòÔÚTÔØÌåÉÏ,Ï뽫ËüÇÐÏÂÀ´Éϱí´ïÔØÌå,ÓõÄøÊÇ¿ìËÙÁ¬½ÓøŶ,ÕâÊDz»ÊÇÓÐÎÊÌâ?
ÄÑÃâÂñԹʱ¼äµÄÊÖ£¬°ÑÏమд³ÉÏà°®¹ý
16Â¥2011-05-10 16:12:57
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yoyoyoyo3985

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
Originally posted by zhangxn2010 at 2011-05-10 16:07:46:
ÕâÊÇǰ¶Îʱ¼äÒ»Ö±À§ÈÅÎÒµÄÎÊÌ⣬²»¹ýÏÖÔÚ½â¾öÁË¡£
Ê×ÏÈ£¬ÄãµÃ±£Ö¤Á¬½ÓÌåϵÖи÷Ƭ¶ÎDNAµÄÖÊÁ¿£¨°üÀ¨Å¨¶ÈºÍ´¿¶È£©£¬Õâ¸öºÜÖØÒª£¬µ±³õÎÒ¾ÍÊÇÒòΪʹÓÃÁËTBEÇнº£¬¶à×öÁËÁ½¸öÔÂʵÑé¶¼²»³ö½á¹û£¨¹úÄڵĽº»ØÊÕÊÔ¼ÁºÐ×îºÃ ...

лл,ÒÔǰûÊÔ¹ýÈȼ¤
ÄÑÃâÂñԹʱ¼äµÄÊÖ£¬°ÑÏమд³ÉÏà°®¹ý
17Â¥2011-05-10 16:15:19
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ѩɽ·Éºü7233

Ìú¸Ëľ³æ (ÖøÃûдÊÖ)

Ìú¸ËС³æ

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

16¶ÈÁ¬½Ó¹ýÒ¹°É¡£
ÃÎÔÚ·¾ÍÔÚ£¡
18Â¥2011-05-10 17:43:42
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yoyoyoyo3985

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
Originally posted by ѩɽ·Éºü7233 at 2011-05-10 17:43:42:
16¶ÈÁ¬½Ó¹ýÒ¹°É¡£

¿ìËÙÁ´½Óø¹ýÒ¹Á´½ÓÒ²ÊÇûÎÊÌâµÄ°É£¿
ÄÑÃâÂñԹʱ¼äµÄÊÖ£¬°ÑÏమд³ÉÏà°®¹ý
19Â¥2011-05-11 10:09:25
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

³óÅ£1973

Òø³æ (³õÈëÎÄ̳)

1.˫øÇÐʱºò£¬ÒªÃ¿¸öø¶¼µ¥Ã¸ÇÐÒ»´Î£¬ÒÔÈ·ÈÏøÇÐλµãºÍø¶¼Ã»ÎÊÌâ
2.ÉèÖöÔÕÕ
20Â¥2011-06-30 16:54:44
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ yoyoyoyo3985 µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 308Çóµ÷¼Á +11 ÌÈÈôÆð·çÁËÄØ 2026-04-05 11/550 2026-04-05 23:21 by À´¿´Á÷ÐÇÓê10
[¿¼ÑÐ] 315Çóµ÷¼Á +5 £¦123456789 2026-04-05 5/250 2026-04-05 19:55 by nepu_uu
[¿¼ÑÐ] Ò»Ö¾Ô¸Çà¿Æ085500£¬³õÊÔ295·Ö£¬¹«¹²¿Î213·Ö +3 Óöµ½µÄÈËÔ¸Íû¶¼Ä 2026-04-05 3/150 2026-04-05 18:45 by À¶ÔÆË¼Óê
[¿¼ÑÐ] Ò»Ö¾Ô¸Î÷±±Å©ÁÖÐóÄÁר˶336·ÖÇóµ÷¼Á +3 5ourr 2026-04-03 3/150 2026-04-05 10:40 by JOKER0401
[¿¼ÑÐ] ²ÄÁϵ÷¼Á +7 dxyµ÷¼Á 2026-04-04 7/350 2026-04-05 09:15 by İÇï26
[¿¼ÑÐ] Ò»Ö¾Ô¸Ö£´ó0705Çóµ÷¼Á +3 éÙʮһ 2026-04-02 4/200 2026-04-05 00:05 by chongya
[¿¼ÑÐ] ²ÄÁÏ¿ÆÑ§Ó빤³Ìµ÷¼Á +19 ÉîVËÞÉá°É 2026-03-30 20/1000 2026-04-04 22:13 by hemengdong
[¿¼ÑÐ] 280Çóµ÷¼Á +21 ¹¾ààÏþÏþ 2026-04-02 22/1100 2026-04-04 11:12 by Öí»á·É
[¿¼ÑÐ] 265Çóµ÷¼Á +17 ÁÖÉîÎÂÀ½ 2026-04-01 20/1000 2026-04-04 01:09 by userper
[¿¼ÑÐ] 305Çóµ÷¼Á +3 77Qi 2026-04-03 3/150 2026-04-03 23:01 by qzxyhcsy
[¿¼ÑÐ] ¿¼Ñе÷¼Á +3 15615482637 2026-04-03 3/150 2026-04-03 22:50 by ms629
[¿¼ÑÐ] Ò»Ö¾Ô¸Öйú¿ÆÑ§Ôº´óѧ265Çóµ÷¼Á +9 Ìñµ­ye 2026-03-31 10/500 2026-04-03 11:10 by txp1986
[¿¼ÑÐ] 279Çóµ÷¼Á +5 ¸µÎÄÇï 2026-04-02 5/250 2026-04-02 18:10 by ±ÊÂä½õÖÝ
[¿¼ÑÐ] Ò»Ö¾Ô¸ÉϺ£º£Ñó´óѧ083200ʳƷѧ˶£¬Çóµ÷¼Á£¬½ÓÊÜÆäËûרҵ +6 whatÕÅ 2026-04-01 7/350 2026-04-02 16:48 by zzsw+
[¿¼ÑÐ] 266Çóµ÷¼Á +4 ѧԱ97LZgn 2026-04-02 4/200 2026-04-02 13:03 by yulian1987
[¿¼ÑÐ] 08ÉúÎïÓëҽҩר˶³õÊÔ346ÕÒµ÷¼Á +6 dianeeee 2026-04-01 7/350 2026-04-02 08:23 by guoweigw
[¿¼ÑÐ] 296Çóµ÷¼Á +4 Íô£¡£¿£¡ 2026-03-31 7/350 2026-04-01 22:04 by ¿Í¶ûÃÀµÂ
[¿¼ÑÐ] 318Çóµ÷¼Á +8 ÆßÒä77 2026-04-01 8/400 2026-04-01 10:37 by Jaylen.
[¿¼ÑÐ] 326Çóµ÷¼Á +4 áÌáÌ×Ð 2026-03-31 4/200 2026-04-01 09:58 by ÎҵĴ¬Îҵĺ£
[¿¼ÑÐ] һ־ԸʳƷ¿ÆÑ§Ó빤³Ì083200Çóµ÷¼Á +4 XQTJZ 2026-03-30 4/200 2026-03-31 04:10 by fmesaito
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û