24СʱÈÈÃŰæ¿éÅÅÐаñ    

²é¿´: 1668  |  »Ø¸´: 6
±¾Ìû²úÉú 1 ¸ö BioEPI £¬µã»÷ÕâÀï½øÐв鿴
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

tangchaoxi

гæ (СÓÐÃûÆø)


[½»Á÷] ¡¾ÇóÖú¡¿µ°°×ÌáÈ¡

ÇëÎÊÏÂËá»áÓ°ÏìÌáϸ¾úµ°°×Âð£¿ÒòΪÎÒÐèÒª½«ÅàÑø»ùÖеÄÄÑÈÜÁ×ÓÃËáÈܵô²ÅÄܵõ½¾úÌ塣лл¡£ÁíÍ⣬Ìá³öµÄµ°°×¿ÉÒÔÖ±½ÓË«ÏòµçÓ¾Âð£¿
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

» ÇÀ½ð±ÒÀ²£¡»ØÌû¾Í¿ÉÒԵõ½:

²é¿´È«²¿É¢½ðÌù

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

tangchaoxi

гæ (СÓÐÃûÆø)


лл£¡ÅàÑø»ùÖÐÊÇÓÐÁ×Ëá¸Æ£¬ÎÒ¾ÍÊÇÒª¼ÓËá°ÑËü¸øÈÜÁË£¬Ê±¼ä²»³¤£¬ÈÜÁËÖ®ºóÁ¢¼´ÀëÐÄ£¬Óûº³åҺϴϴ¿ÉÒÔÂð£¿ÎÒÊÇÏëÌáµ°°×£¬Ë«ÏòµçÓ¾ÕÒ²îÒìµ°°×¡£Ö±½ÓÉÏË«ÏòÕÒ¿ÉÒÔÂð£¿
3Â¥2011-04-13 08:50:37
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 7 ¸ö»Ø´ð

HarveyWang

¾èÖú¹ó±ö (ÖªÃû×÷¼Ò)


¡ï ¡ï
amisking(½ð±Ò+2, EPI+1): ¹ÄÀø·¢Ìû½»Á÷¡£ 2011-04-12 21:45:46
tangchaoxi(½ð±Ò+3): 2011-04-13 08:45:56
ÒýÓûØÌû:
Originally posted by tangchaoxi at 2011-04-12 11:18:00:
ÇëÎÊÏÂËá»áÓ°ÏìÌáϸ¾úµ°°×Âð£¿ÒòΪÎÒÐèÒª½«ÅàÑø»ùÖеÄÄÑÈÜÁ×ÓÃËáÈܵô²ÅÄܵõ½¾úÌ塣лл¡£ÁíÍ⣬Ìá³öµÄµ°°×¿ÉÒÔÖ±½ÓË«ÏòµçÓ¾Âð£¿

ºóÃæ¿´µ½£¬ÄãµÄµ°°×ÊÇÓÃÀ´×öË«ÏòµçÓ¾µÄ£¬¶ø²»ÊDzâ»îµÄ¡£
Òò´Ë£¬µÍpHµÄËáÓ¦¸ÃÓ°Ïì²»µ½µ°°×°É¡£ ¹Ø¼üÊÇÄã¼ÓËáÁ¿ÓжàÉÙ£¿
ÄѵÀÒª¼Ó100 mMµÄHCl´¦Àí һСʱ£¿
ÄǾúÌå¹À¼Æ¶¼ÒªÆÆµôÁË¡£


ÄãµÄ¾úÌåµÄ»ñµÃºÜÂé·³Âð£¿ÄѵÀÊÇÉú³¤ÔÚÁ×Ëá¸ÆÖ®ÀàµÄÉÏÃæ£¿


¾úÌåÌáÈ¡µÄµ°°×£¬Òª·Ö³É¼¸Àࣺ
1£©×î¼òµ¥µÄ£¬¿ÉÈÜÐÔÉÏÇåÖеĵ°°×: È¥³ýÑÎÀàºó£¬¿ÉÒÔ×öË«ÏòµçÓ¾µÄÑùÆ·¡£

2£©Ä¤µ°°×£¬ÒòΪ¿ÉÈÜÐԲÐèÒªÌØÊâµÄÊÔ¼Á´¦Àí£¬Ôö¼ÓÆäÔÚµÚһάµçӾʱµÄ¿ÉÈÜÐÔ¡£¾ßÌåµÄÄãµÃÈ¥²éÂÛÎÄ£¬ÕâÀïÖ»ÊÇÌáʾһÏ¡£

ÁíÍ⣬ʵ¼ÊÉÏ£¬¶ÔÓÚȫϸ°ûµ°°×£¬Ë«ÏòµçÓ¾Ðí¶àʱºò»á¹ýÂ˵ôºÜ¶àµ°°×µãµÄ£¡
Ñ¡ÔñË«ÏòµçÓ¾Loading buffer ×é·ÖºÜ¹Ø¼ü¡£
2Â¥2011-04-12 17:41:10
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

tangchaoxi

гæ (СÓÐÃûÆø)


ÒýÓûØÌû:
Originally posted by HarveyWang at 2011-04-12 17:41:10:
ºóÃæ¿´µ½£¬ÄãµÄµ°°×ÊÇÓÃÀ´×öË«ÏòµçÓ¾µÄ£¬¶ø²»ÊDzâ»îµÄ¡£
Òò´Ë£¬µÍpHµÄËáÓ¦¸ÃÓ°Ïì²»µ½µ°°×°É¡£ ¹Ø¼üÊÇÄã¼ÓËáÁ¿ÓжàÉÙ£¿
ÄѵÀÒª¼Ó100 mMµÄHCl´¦Àí һСʱ£¿
ÄǾúÌå¹À¼Æ¶¼ÒªÆÆµôÁË¡£


ÄãµÄ¾úÌåµÄ»ñµÃºÜÂé·³ ...

ÎÒÖ±½ÓÓÃŨÑÎËáÈÜ£¬¿ÉÒÔÂð
4Â¥2011-04-13 09:00:21
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

HarveyWang

¾èÖú¹ó±ö (ÖªÃû×÷¼Ò)


tangchaoxi(½ð±Ò+3): 2011-04-13 20:28:37
ÒýÓûØÌû:
Originally posted by tangchaoxi at 2011-04-13 09:00:21:
ÎÒÖ±½ÓÓÃŨÑÎËáÈÜ£¬¿ÉÒÔÂð

¾ø¶Ô²»¿ÉÒÔ¡£
ŨÑÎËá»áÈܽâϸ°û£¬1min ÄÚ¾ÍÈܽâÁË£¬È»ºó´ó²¿·Öµ°°×±»Ëá³Á¡£
Ëᣬ¼´Ê¹50 mM HCl£¬Ò²»á³ÁµíÐí¶àϸ°ûµ°°×¡£

ÎÒ¾õµÃÄãû±ØÒª·ÇÒªÕâʱºòÈ¥³ýÁ×Ëá¸Æ¡£
Äã¿ÉÒÔÔÚÆÆËé¾úÌåʱÀëÐÄÈ¥³ýµÄ¡£


ÄãÈç¹ûÊÇ×ö¾úÌåÉÏÇåµ°°×£¬¶ø·¢½ÍҺûÓÐÆäËû¹ÌÌåÅàÑø»ùµÄ»°£¬
½¨ÒéÄãÔÚpH8-10Ö®¼ä£¬Ö±½Ó³¬ÉùÆÆËé  ·¢½ÍÒº³Áµí£¨¾ÍÊÇÁ×Ëá¸Æ+¾úÌ壩£¬
È»ºóÔÚ4¡æ 10000 rpm  ÀëÐÄ10-20min,
ÕâÑù£¬Á×Ëá¸Æ¶¼³ÁµíÁË£¬  È¡ÉÏÇå¼´¿É¡£

Èç¹ûÏëÈܽâ¸ü¶àµÄĤµ°°×£¨»òÈ«¾úÌåµ°°×£©£¬ÐèÒªÓÃ2DµçÓ¾µÄLoading Buffer ¼ÓÈë·¢½ÍÒº³Áµí£¬À´Ö±½Ó³¬ÉùÆÆËé³Áµí£¬È»ºóÔÚ4¡æ 10000 rpm  ÀëÐÄ10-20min£¬È¡ÉÏÇå¡£

Èç¹ûÏë¼ø¶¨ÆÆËéÒºµÄµ°°×£¬ ¾ÍÓÃSDS -PAGEµçÓ¾ loading buffer, ¼ÓÈëÑùÆ·ºó·Ðˮԡ3-5min£¬ÀëÐÄ£¬ÉÏÑù¼´¿É¡£

Äã¿´ÈçºÎ£¿

[ Last edited by HarveyWang on 2011-4-13 at 18:40 ]
5Â¥2011-04-13 18:34:09
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] Çó²ÄÁϵ÷¼Á +8 ¸ô±Ú³ÂÏÈÉú 2026-03-12 8/400 2026-03-18 22:19 by li123456789.
[¿¼ÑÐ] 344Çóµ÷¼Á +6 knight344 2026-03-16 7/350 2026-03-18 20:13 by walc
[¿¼ÑÐ] ¡¾Í¬¼ÃÈí¼þ¡¿Èí¼þ£¨085405£©¿¼ÑÐÇóµ÷¼Á +3 2026eternal 2026-03-18 3/150 2026-03-18 19:09 by ²«»÷518
[½Ìʦ֮¼Ò] ½¹ÂÇ +8 Ë®±ùÔÂÔÂÒ°Íà 2026-03-13 12/600 2026-03-18 15:27 by ßäÎØß÷ÎØ
[¿¼ÑÐ] 311Çóµ÷¼Á +6 26ÑÐ0 2026-03-15 6/300 2026-03-18 14:43 by haxia
[¿¼ÑÐ] 0854¿É¿çµ÷¼Á£¬Ò»×÷Ò»ÏîºËÐÄÂÛÎÄÎåÏîרÀû£¬Ê¡¡¢¹ú¼¶Ö¤Êé40+ÊýÒ»Ó¢Ò»287 +8 СÀî0854 2026-03-16 8/400 2026-03-18 14:35 by ²«»÷518
[¿¼ÑÐ] 280Çóµ÷¼Á +6 ¹¾ààÏþÏþ 2026-03-18 7/350 2026-03-18 11:25 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] ²ÄÁÏ£¬·ÄÖ¯£¬ÉúÎ0856¡¢0710£©£¬»¯Ñ§ÕÐÉúÀ² +3 Eember. 2026-03-17 9/450 2026-03-18 10:28 by Eember.
[¿¼ÑÐ] 307Çóµ÷¼Á +3 ÀäóÏ123 2026-03-17 3/150 2026-03-18 09:55 by macy2011
[¿¼ÑÐ] 301Çóµ÷¼Á +9 yyÒªÉϰ¶Ñ½ 2026-03-17 9/450 2026-03-18 08:58 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 290Çóµ÷¼Á +6 ¿×Ö¾ºÆ 2026-03-12 11/550 2026-03-17 14:41 by ÖÜÖÛÖÛ77
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤×¨Ë¶µ÷¼Á +5 heming3743 2026-03-16 5/250 2026-03-17 14:03 by Ó¸ÒÌ«¼àÍõ¹«¹«
[¿¼ÑÐ] »úеר˶325£¬Ñ°ÕÒµ÷¼ÁԺУ +3 y9999 2026-03-15 5/250 2026-03-16 19:58 by y9999
[¿¼ÑÐ] 326Çóµ÷¼Á +4 ŵ±´¶û»¯Ñ§½±êéê 2026-03-15 7/350 2026-03-16 17:11 by ŵ±´¶û»¯Ñ§½±êéê
[¿¼ÑÐ] 0703Ò»Ö¾Ô¸211 285·ÖÇóµ÷¼Á +5 ly3471z 2026-03-13 5/250 2026-03-16 16:16 by ŶŶ123
[¿¼ÑÐ] 277²ÄÁÏ¿ÆÑ§Ó빤³Ì080500Çóµ÷¼Á +3 ×ÔÓɼå±ý¹û×Ó 2026-03-16 3/150 2026-03-16 14:10 by ÔËÆøyunqi
[¿¼ÑÐ] ²ÄÁÏ080500µ÷¼ÁÇóÊÕÁô +3 Ò»¿Åmeteor 2026-03-13 3/150 2026-03-14 10:54 by peike
[¿¼ÑÐ] ²ÄÁÏר˶350 Çóµ÷¼Á +4 Íõ½ð¿Æ 2026-03-12 4/200 2026-03-13 16:02 by ruiyingmiao
[¿¼ÑÐ] Çóµ÷¼Á +3 ³ÌÓ꺼 2026-03-12 3/150 2026-03-13 15:06 by JourneyLucky
[¿¼ÑÐ] һ־Ըɽ´ó07»¯Ñ§ 332·Ö ËÄÁù¼¶Òѹý ±¾¿ÆÉ½¶«Ë«·Ç Çóµ÷¼Á£¡ +3 ²»ÏëÀíÄã 2026-03-12 3/150 2026-03-13 14:18 by JourneyLucky
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û