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The S0-oxidizing bacterial consortium capable of reducing ClO4 to Cl was enriched from municipal activated sludge. The culture was enriched in 300 mL serum bottles. A volume of 6 mL activated sludge sample (5% v/v) was added to 120 mL of culture medium containing 3 mMperchlorate, 20 mM S0 powder, and basal medium amended with trace metal solution I and YE. After purging the headspace with N2/CO2 (80:20, v/v), the enrichment cultures were incubated in the dark at 308C on a shake table (100 rpm). When ClO4 was depleted, the culture was transferred again by inoculating enriched culture (5%, v/v) to fresh medium. The culture was transferred every 21–25 days. 大家帮忙，看看，我就感觉如果每个富集周期结束以取5%的体积，开始下一个富集周期，感觉不太合理。想问问大家正确理解是这样吗？ 注：ClO4——高氯酸根。Cl——氯离子。S0——硫单质。 |
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