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北京石油化工学院2026年研究生招生接收调剂公告
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vs570588

木虫 (正式写手)


[交流] 求翻译一段英文

The S0-oxidizing bacterial consortium capable of reducing
ClO4 to Cl was enriched from municipal activated sludge.
The culture was enriched in 300 mL serum bottles. A
volume of 6 mL activated sludge sample (5% v/v) was added
to 120 mL of culture medium containing 3 mMperchlorate,
20 mM S0 powder, and basal medium amended with
trace metal solution I and YE. After purging the headspace
with N2/CO2 (80:20, v/v), the enrichment cultures were
incubated in the dark at 308C on a shake table (100 rpm).
When ClO4 was depleted, the culture was transferred again
by inoculating enriched culture (5%, v/v) to fresh medium.
The culture was transferred every 21–25 days.
大家帮忙,看看,我就感觉如果每个富集周期结束以取5%的体积,开始下一个富集周期,感觉不太合理。想问问大家正确理解是这样吗?
注:ClO4——高氯酸根。Cl——氯离子。S0——硫单质。

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vs570588

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引用回帖:
Originally posted by zxs1989 at 2011-04-03 22:31:08:
难道不是培养纯菌吗?
“The S0-oxidizing bacterial consortium capable of reducing ClO4 to Clwas enriched from municipal activated sludge.”
作者的本意应该就是富集培养硫氧化菌啊!!

是的。那就这样了。他这种驯化加入了一定量的有机物。有可能微生物生长很快。我们实际做是完全自养,生长很慢。所以又可能能讲的通
8楼2011-04-04 09:05:24
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seaharrier

铁杆木虫 (知名作家)


引用回帖:
Originally posted by vs570588 at 2011-03-31 09:50:38:
The S0-oxidizing bacterial consortium capable of reducing
ClO4 to Cl was enriched from municipal activated sludge.
The culture was enriched in 300 mL serum bottles. A
volume of 6 mL activated  ...

不对吧
2楼2011-03-31 13:40:32
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vs570588

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引用回帖:
Originally posted by seaharrier at 2011-03-31 13:40:32:
不对吧

那你说是啥意思
3楼2011-03-31 18:47:13
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zxs1989

金虫 (著名写手)


“A volume of 6 mL activated sludge sample (5% v/v) was added to 120 mL of culture medium”
“When ClO4 was depleted, the culture was transferred again by inoculating enriched culture (5%, v/v) to fresh medium.”

是这样。培养基是120 mL, 5%体积比是 6 mL。最初接种的活性污泥也是 6 mL,进一步富集硫氧化菌时也应该用 6 mL, 也就是5%体积比。
4楼2011-04-02 21:58:56
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