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北京石油化工学院2026年研究生招生接收调剂公告
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vs570588

木虫 (正式写手)


[交流] 求翻译一段英文

The S0-oxidizing bacterial consortium capable of reducing
ClO4 to Cl was enriched from municipal activated sludge.
The culture was enriched in 300 mL serum bottles. A
volume of 6 mL activated sludge sample (5% v/v) was added
to 120 mL of culture medium containing 3 mMperchlorate,
20 mM S0 powder, and basal medium amended with
trace metal solution I and YE. After purging the headspace
with N2/CO2 (80:20, v/v), the enrichment cultures were
incubated in the dark at 308C on a shake table (100 rpm).
When ClO4 was depleted, the culture was transferred again
by inoculating enriched culture (5%, v/v) to fresh medium.
The culture was transferred every 21–25 days.
大家帮忙,看看,我就感觉如果每个富集周期结束以取5%的体积,开始下一个富集周期,感觉不太合理。想问问大家正确理解是这样吗?
注:ClO4——高氯酸根。Cl——氯离子。S0——硫单质。

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vs570588

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引用回帖:
Originally posted by zxs1989 at 2011-04-02 22:00:35:
我是环境工程专业的,不过现在不接触微生物,可能有理解不对的地方,希望对你有帮助。

你的意思也就是每开始一个富集驯化周期,就取上一个周期6mL作为下一个周期接种液。文章中说降解污染物的速率不断增加。根据富集越来越成熟,可以讲通,但是我还是认为,每一次开始新的富集,就相当于用新鲜的富集培养液把上一个周期富集培养液稀释了几乎20倍。感觉是在富集驯化纯菌。而不是混合培养液。
6楼2011-04-03 18:32:34
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seaharrier

铁杆木虫 (知名作家)


引用回帖:
Originally posted by vs570588 at 2011-03-31 09:50:38:
The S0-oxidizing bacterial consortium capable of reducing
ClO4 to Cl was enriched from municipal activated sludge.
The culture was enriched in 300 mL serum bottles. A
volume of 6 mL activated  ...

不对吧
2楼2011-03-31 13:40:32
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vs570588

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引用回帖:
Originally posted by seaharrier at 2011-03-31 13:40:32:
不对吧

那你说是啥意思
3楼2011-03-31 18:47:13
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zxs1989

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“A volume of 6 mL activated sludge sample (5% v/v) was added to 120 mL of culture medium”
“When ClO4 was depleted, the culture was transferred again by inoculating enriched culture (5%, v/v) to fresh medium.”

是这样。培养基是120 mL, 5%体积比是 6 mL。最初接种的活性污泥也是 6 mL,进一步富集硫氧化菌时也应该用 6 mL, 也就是5%体积比。
4楼2011-04-02 21:58:56
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