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[交流] 【求助/交流】怎样用谷胱甘肽单乙酯（GSH-OEt）对细胞进行预处理，增加细胞内谷胱甘肽

请问各位前辈，怎样用谷胱甘肽单乙酯（GSH-OEt）对细胞进行预处理，增加细胞内谷胱甘肽的浓度？谢谢！

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1楼 2011-03-21 20:03:29

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6楼: Originally posted by pjgwade at 2012-04-06 19:19:44
楼主的单乙酯价格请告诉我急求谢谢！！！

GSH-OEt购买的话，SIGMA是600多元25mg,1860元500mg,稍贵。
以下为其简便合成方法。
原来看到有人在求助，GSH-OEt哪里买？或者说怎么合成。
合成过程如下，和大家分享。
A freshly prepared solution of hydrogen
chloride (16 g, 0.45 mol) in 250 ml of
anhydrous ethanol was cooled to 20°C in
an ice bath, and 20 g (65 mmol) of glutathione
was added and dissolved by gentle
shaking. The mixture was allowed to stand
at 20°C for 2.5 h, during which time the
progress of the reaction was followed by
thin-layer chromatography (see Materials
and Methods). The solution was then
cooled to 0°C and 1.8 liter of ice-cold, dry
diethyl ether was added. The precipitate
which formed was allowed to settle for l-
3 h at 0°C. The ether was decanted and
the precipitate was washed twice with 500
ml of ice-cold ether. The product, after
drying in vacua over CaClz and KOH, was
obtained in 98% yield. The content of
glutathione monoethyl ester found on
amino acid analysis (see Materials and
Methods) varied from 90 to 98%; the other
components were glutathione and glutathione
diethyl ester. The preparations
obtained typically contained O-5% glutathione.

文献

Glutathione monoethyl ester: Preparation, uptake by tissues, and conversion to glutathione
Archives of biochemistry and biophysics
1985 Jun
239(2):538-48.